After years of exploration in the field of antibody discovery and immunotherapy, Creative Biolabs now holds an outstanding platform which can provide you professional and excellent service for T Cell Receptor (TCR) related therapy purpose. This service enables higher TCR affinity mature level for much efficient results in further research.
Affinity maturation, which is a complex process in immunology, usually happens on surface immunoglobulin of B cells, as well as a direct result of somatic hypermutation (SHM) and selection by Tfh cells. TCR is a molecule existed on the surface of T cells, or T lymphocytes. It functions by recognization of MHC/peptide complex. TCR affinity maturation process can give out an option with much greater affinity, which will bring about positive influence in immune application.
TCR Affinity Maturation Service
We have successfully operated a platform for affinity maturation service, which can especially be used for TCR. Some vital methods/steps are as follows:
If the structure of the antibody/antigen complex is available, certain positions can be disturbed at a defined diversity to improve the affinity. We will work on the sequences of the antibodies to find out the conserved sequences and then introduce mutations to the positions in the framework regions that are not conserved.
Subsequent library screening will fish out the mutants that have high affinity. Two library screening strategies are available. In the first strategy, surface-panning, decreasing concentrations of antigen is surface immobilized. In the second, solution-sorting, strategy, a labeled antigen in solution will be used.
We offer binding kinetic analyses of antibodies and capture the antibody on chips and run antigen on top of the captured antibody.
Alanine scanning mutagenesis is the most popular method in affinity maturation of peptide binders. The non-essential AAs will be specifically identified. We will then create a directed/constrained peptide sub-library that contains random sequences on the non-essential AA positions. Finally, we frequently randomize the non-essential residues using special strategy.
Highlight Features of Our Service
Creative Biolabs is in the leading position in the field of immunotherapy with numerous experiences. We currently provide professional and advanced service to customers around the world, giving them assistance in time for each project. It’s our responsibility to promote your projects and boost the progress to clinical trials with the help of our advanced technologies, platforms, and resources. For more details, please feel free to contact us. We will reply to you as soon as possible.
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