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TCR Affinity Maturation Service

After years of exploration in the field of antibody discovery and immunotherapy, Creative Biolabs now holds an outstanding platform which can provide you professional and excellent service for T Cell Receptor (TCR) related therapy purpose. This service enables higher TCR affinity mature level for much efficient results in further research.

Background

Affinity maturation, which is a complex process in immunology, usually happens on surface immunoglobulin of B cells, as well as a direct result of somatic hypermutation (SHM) and selection by Tfh cells. TCR is a molecule existed on the surface of T cells, or T lymphocytes. It functions by recognization of MHC/peptide complex. TCR affinity maturation process can give out an option with much greater affinity, which will bring about positive influence in immune application.

TCR Affinity Maturation Service

TCR Affinity Maturation Service

We have successfully operated a platform for affinity maturation service, which can especially be used for TCR. Some vital methods/steps are as follows:

TCR Affinity Maturation Service

  1. Untargeted Mutagenesis
    We use an error-prone PCR integrated DNA-shuffling method to mutate main CDR regions during sub-library construction. If the potential of introducing immunogenic mutations to framework positions is given, we can provide the approach to create mutations at completely random positions.


  2. Oligonucleotide-directed Mutagenesis

    If the structure of the antibody/antigen complex is available, certain positions can be disturbed at a defined diversity to improve the affinity. We will work on the sequences of the antibodies to find out the conserved sequences and then introduce mutations to the positions in the framework regions that are not conserved.

  3. Phage Display Antibody Library Screening

    Subsequent library screening will fish out the mutants that have high affinity. Two library screening strategies are available. In the first strategy, surface-panning, decreasing concentrations of antigen is surface immobilized. In the second, solution-sorting, strategy, a labeled antigen in solution will be used.

  4. Antibody Affinity Measurement

    We offer binding kinetic analyses of antibodies and capture the antibody on chips and run antigen on top of the captured antibody.

  5. Peptide Affinity Maturation

    Alanine scanning mutagenesis is the most popular method in affinity maturation of peptide binders. The non-essential AAs will be specifically identified. We will then create a directed/constrained peptide sub-library that contains random sequences on the non-essential AA positions. Finally, we frequently randomize the non-essential residues using special strategy.

Highlight Features of Our Service

  • Ph.D. level scientists and skillful technicians
  • Extensive experience
  • Superior quality

Creative Biolabs is in the leading position in the field of immunotherapy with numerous experiences. We currently provide professional and advanced service to customers around the world, giving them assistance in time for each project. It’s our responsibility to promote your projects and boost the progress to clinical trials with the help of our advanced technologies, platforms, and resources. For more details, please feel free to contact us. We will reply to you as soon as possible.

Online Inquiry

For any technical issues or products/services related questions, please leave your contacts as below, and our team will contact you at earliest convenience to let you know how we can be involved in your projects.

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