Tumor-infiltrating Lymphocyte Penotype Identification Service

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Creative Biolabs is at the forefront of CIK therapy, dedicated to providing high-quality in vitro phenotype identification services for tumor-infiltrating lymphocytes (TILs). Utilizing a high-throughput technology platform, we precisely analyze the phenotypic characteristics of TILs and achieve detailed classification and assessment of various subpopulations. Additionally, through a comprehensive analysis of TILs' functionality, cell markers, and immune properties, we provide reliable data to assist our clients in evaluating or optimizing their CIK treatment strategies.

Tumor-infiltrating Lymphocyte Phenotype Identification Service at Creative Biolabs

The tumor microenvironment is intricate and variable, and the composition and function of TILs may directly influence the effectiveness of CIK therapy. By performing phenotype identification services, Creative Biolabs can confirm the specific subgroups of TILs, allowing for an assessment of their immune response capabilities against the tumor. Furthermore, through our phenotypic analysis, we can predict the prognosis of CIK therapy and select which types of tumors are more susceptible to this therapy, ultimately enhancing the chances of successful therapy outcomes.

At Creative Biolabs, we have used flow cytometry and immunohistochemistry to classify TIL subpopulations across various tumor types, identifying specific subsets such as CD8+ cytotoxic T lymphocytes and regulatory T cells, whose ratios are closely linked to cancer prognosis. Meanwhile, the use of multiplex immunofluorescence labeling technology allows us for precise analysis of the spatial distribution and functional status of TILs. Moreover, by enhancing the antitumor activity of TILs through phenotypic characterization, we can also provide potential biomarkers and targets to optimize cancer immunotherapy.

Identifying Tumor Infiltrating Lymphocyte (TIL) Tegions in Gigapixel Pathology WSIs. Fig.1 Tumour-infiltrating Lymphocytes and Identification.^1,3

Our Service Workflows

STEP1: Sample Collection

Tumor tissues are obtained from various types of tumors to ensure representative samples are collected, adhering strictly to ethical guidelines.

STEP2: Cell Isolation

Enzymatic digestion techniques, such as using collagenase and DNase, are employed to process tumor tissues, facilitating the separation of tumor cells from infiltrating lymphocytes.

STEP3: Cell Culture

The isolated TILs are cultured in specific media designed to promote their proliferation and activity. Cytokines, such as IL-2 and IL-7, may be added to enhance cell viability.

STEP4: Surface Marker Analysis

Flow cytometry is used to identify surface markers on TILs, labeling specific antigens (such as CD3, CD4, CD8) to distinguish various T cell populations. Multiplex flow cytometry allows for the simultaneous detection of multiple markers, enabling detailed analysis of TIL subsets.

STEP5: Functional Analysis

The functional status of TILs is assessed through cytotoxicity assays or cytokine secretion experiments to measure their capacity to kill tumor cells. ELISA or colorimetric assays can be employed to evaluate the secretion of cytokines (such as IFN-γ, and TNF-α).

STEP6: Gene Expression Profiling

Single-cell RNA sequencing is conducted on TILs to analyze their gene expression profiles, providing insights into their active states and regulatory mechanisms. Comparative analysis of gene expression differences among TILs from various patients or tumor types offers a foundation for personalized therapy approaches.

Multiplex Immunofluorescence Assessment of Different TIL Subsets in the Tumour Microenvironment. Fig.2 Immunofluorescence Analysis of Various Tumor-Infiltrating Lymphocyte Populations.^2,3

Why Choose Us

High-Throughput Testing: Using cutting-edge technologies, Creative Biolabs can conduct rapid and efficient analyses of large sample volumes, ensuring the reliability and reproducibility of research data.
Multidimensional Information Integration: By combining analyses of cell phenotypes, functional states, and secreted factors, we provide a comprehensive evaluation of TILs' immune activity, offering multifaceted data support for scientific research.
Wide Applicability: Our services cater to various types of cancer research, including solid tumors and hematological malignancies, thereby addressing diverse research needs.
Immune Microenvironment Analysis: By thoroughly investigating the distribution and status of TILs within the tumor microenvironment, we can assist clients in uncovering mechanisms of tumor immune evasion, thereby providing a foundation for exploring new therapeutic targets.
Facilitating Biomarker Discovery: Through the phenotypic analysis of TILs, our team can identify potential biomarkers, providing new insights for early diagnosis and treatment.

Currently, TILs play a crucial role within the tumor microenvironment, and their phenotypic characterization can provide valuable insights for immunotherapy, prognosis assessment, and personalized treatment approaches. Creative Biolabs is equipped with advanced flow cytometry and high-throughput sequencing technologies, enabling comprehensive and precise phenotyping of TILs. With our specialized TIL phenotyping services, you will gain a deeper understanding of tumors and receive a more targeted CIK therapy plan. If you are interested in our services or have any questions, please feel free to contact us at any time.

References

Abousamra, Shahira, et al. "Deep learning-based mapping of tumor-infiltrating lymphocytes in whole slide images of 23 types of cancer." Frontiers in oncology 11 (2022): 806603.
Salas-Benito, Diego, et al. "The mutational load and a T-cell inflamed tumor phenotype identify ovarian cancer patients rendering tumor-reactive T cells from PD-1+ tumor-infiltrating lymphocytes." British journal of cancer 124.6 (2021): 1138-1149.
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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.