3D Hanging Spheroid Plate based Cytotoxicity Assay Service

Are you encountering technical challenges in modeling the complex tumor microenvironment (TME) of solid tumors? Conventional two-dimensional (2D) cell culture models often fail to provide reliable data due to their lack of physiological relevance, leading to predictive inaccuracies and prolonged research cycles. To address this issue, Creative Biolabs' 3D Hanging Spheroid Plate-based Cytotoxicity Assay Service leverages innovative microplate technology to enable stable and reproducible generation of uniform spheroids. This platform offers a more accurate and efficient in vitro model, significantly accelerating drug discovery and facilitating high-throughput therapy screening.

Introduction

Cell culture underpins modern life-science research, yet conventional two-dimensional monolayers on rigid substrates inadequately reconstruct the biochemical and biophysical complexity of native tissue. This limitation is particularly acute for solid neoplasms, where TME, a highly organized and heterocellular ecosystem, governs therapeutic response. Three-dimensional (3D) tumor spheroids, by recapitulating key TME features such as nutrient gradients, hypoxic cores, cell-cell and cell-matrix interactions, and gene-expression profiles akin to in vivo tumors, have therefore become a more physiologically relevant model for evaluating drug responses and developing cell-based therapies.

Fig.1 Schematic of the 3D hanging-drop spheroid platform for cytotoxicity assessment.¹

3D Hanging Spheroid Plate-based Cytotoxicity Assay Service at Creative Biolabs

Creative Biolabs' 3D hanging spheroid plate-based cytotoxicity assay service provides a specialized cell culture platform designed for cultivating and maintaining 3D cellular spheroids. This system enables improved simulation of the in vivo microenvironment, facilitating the investigation of intricate cell-cell and cell-matrix interactions. We support a wide range of applications, from basic biological research to drug discovery and personalized medicine.

What we can offer

Our 3D hanging spheroid plate cytotoxicity assay service employs state-of-the-art suspended droplet methodology to generate and culture uniform 3D spheroids. These structures are systematically exposed to cytotoxic agents to evaluate their impact on cellular viability.

Our Service Process

We deliver end-to-end support, from initial spheroid formation and compound treatment through to rigorous data interpretation and reporting, ensuring robust and actionable results for you.

Required Starting Materials:

• Target cell lines.
• The therapeutic agents for testing, such as specific CAR-T cells or antineoplastic drugs.

Key Steps Involved:

Final Deliverables: Upon completion, you will receive a comprehensive data package including dose-dependent efficacy curves, live/dead cell counts, and a final technical report with a detailed analysis of the results.

Key Advantages

• Simplified Workflow and Data Accuracy: Our unique hanging drop spheroid-filter plate simplifies the assay by efficiently separating unbound and dead cells, which minimizes manual steps and ensures more accurate and reliable quantitative data.
• Kinetic and Quantitative Analysis: We utilize advanced image cytometry to provide a kinetic evaluation of cytotoxic potency, delivering time- and dose-dependent results that go beyond simple endpoint measurements.
• Robust Quality Control: Our processes are supported by rigorous quality control measures, ensuring the stability of your cell lines throughout the assay and providing you with dependable, reproducible results.

FAQ

Why Choose Us?

Creative Biolabs offers a notable advancement in cell-based research. Integrating a physiologically relevant 3D model with an efficient high-throughput workflow, our service delivers accurate and reliable data to support critical research decisions. It is designed to accelerate project timelines, reduce resource expenditure, and ultimately enhance the success of your investigative and therapeutic endeavors.

Customer Reviews

"Using Creative Biolabs' 3D Hanging Spheroid Plate-based Cytotoxicity Assay Service in our research has significantly improved the physiological relevance of our in vitro models. The data we get is far more predictive than what we saw with 2D culture, and it's a critical step toward validating our drug candidates before in vivo studies." J**n D****e.

"The hanging drop design and the ability to easily separate dead and unbound cells is a game-changer. We were able to run high-throughput screens efficiently and with much less manual work, which saved us a lot of time compared to our previous methods." Sh K*.

"The data from the service, particularly the dose-response curves and the viability-area correlation, has given us the quantitative evidence we needed to move forward with our project." My H***s.

How to contact us?

Reach out to our specialists to explore how our expertise can support your specific project needs. We are ready to collaborate with you to overcome your research challenges using our innovative technologies.

Unlock Your Project's Potential with Creative Biolabs – Request a Quote Today.

Reference

1. Chen, Zhenzhong et al. "3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay." Journal of nanobiotechnology vol. 20,1 30. Distributed under Open Access License CC BY 4.0, without modification. https://doi.org/10.1186/s12951-021-01213-8.