Anti-Fetal AChR (mAb198) ICD(CD86+FcγRI) CAR THP1 Cell Line (CARTHP-W0698)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-Fetal AChR chimeric antigen receptor (CAR) THP1 cell line is a stable cell line developed using anti-Fetal AChR CAR lentivirus transfection. This transfected CAR lentiviral vector is constructed to express a scfv of anti-Fetal AChR antibody (mAb198) linked to CD86+FcγRI signaling domains.The anti-Fetal AChR CAR engineered THP1 cells can be used to be differentiated into macrophage cells for in vitro or in vivo phagocytosis studies. This CAR-THP1 cell line product can be used for the researches of Osteosarcoma, rhabdomyosarcoma.

Specific Inquiry

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  • Marker:
  • Positive rate:
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Details

  • Target
  • Fetal AChR
  • Cell Type
  • THP1
  • Cell Background
  • THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities. CAR engineered THP1 cells can be differentiated into macrophages using PMA (Phorbol 12-myristate 13-acetate). Once differentiated (M0 macrophages), they were incubated with IL-4 and IL-13 in order to obtain M2 polarized macrophages or with IFN-gamma and LPS for classical macrophage activation (M1).
  • Targeting Diseases
  • Osteosarcoma, rhabdomyosarcoma
  • CAR Expression
  • Positive
  • CAR Positive Rate
  • >95% for stable cell pool (please submit an inquiry if single clonal cell line is needed.)
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • Negative
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Safety Consideration
  • Biosafety Level 1
  • Restriction
  • Research use only
  • Size
  • 2 million cells*2 vials
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • mAb198
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • CD86+FcγRI
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(CD86+FcγRI)
  • Discription of Signaling Cassetes
  • The cytoplasmic portion of the human CD86 (amino acids 269-329) plus the cytoplasmic part of the human FcγRI (amino acids 314-374) are used as the CAR intracellular signaling domains.
    CD86:
    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.

    FcγRI:
    This protein is a high-affinity Fc-gamma receptor, and plays an important role in the immune response. As a member of the immunoglobulin superfamily (it has two Ig-like domains), FcγRI is expressed on macrophage and other cells, controlling the production of important immune mediators called cytokines that promote inflammation. Fc gamma receptors belong to the group of non-catalytic tyrosine-phosphorylated receptors which share a similar signalling pathway involving phosphorylation of tyrosine residues. The receptors generate signals within their cells through an important activation motif known as an immunoreceptor tyrosine-based activation motif (ITAM). An ITAM is present in the intracellular tail of FcγRIIA, and its phosphorylation induces phagocytosis in macrophages. FcγRI and FcγRIIIA do not have an ITAM but can transmit an activating signal to their phagocytes by interacting with another protein that does. This adaptor protein is called the Fcγ subunit and, like FcγRIIA, contains the two YXXL sequences that are characteristic of an ITAM.

Target

  • Target
  • Fetal AChR
  • Target Species
  • Human
  • Clone
  • mAb198
  • Host
  • Rat
  • Gene Name
  • Fetal acetylcholine receptor
  • Introduction
  • This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • Alternative Names
  • FADS; CMS11; CMS4C; FADS2; RAPSYN; RNF205; RAPSN; Receptor associated protein of the synapse; fetal AChR

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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