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Anti-VEGFR2 (4F1)-CD147 CAR-MA (CARMA-W1519)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-VEGFR2 chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human VEGFR2 and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-VEGFR2 antibody (4F1) linked to CD147 signaling domains. The vector product was designed for the treatment of Melanoma, brain cancer.

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Details

  • Target
  • VEGFR2
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Melanoma, brain cancer
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • 4F1
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • VEGFR2
  • Target Species
  • Human
  • Clone
  • 4F1
  • Host
  • Mouse
  • Gene Name
  • Kinase insert domain receptor
  • Introduction
  • Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
  • Alternative Names
  • FLK1; CD309; VEGFR; VEGFR2; KDR; kinase insert domain receptor

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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