Cytokine Analysis by ELISA or CBA

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Conventional ELISA or Cytometric Bead Array (CBA) are commonly used in immunology to measure cytokines secreted from cells. With advanced technology platforms and extensive expertise, Creative Biolabs is dedicated to developing optimized cytokine analysis services for our global clients.

ELISA is a useful technique that is suitable for studies with relatively few samples and interest targets detected. Meanwhile, this method is fairly sensitive in detecting concentrations of samples with the ability to detect concentrations as low as 5 pg/mL.

Comparing to ELISA, the CBA (Cytometric Bead Array) is a convenient and powerful technique. This method is highly sensitive and can detect analytes concentration as low as 2 pg/mL. At the same time, multiple interest cytokines can be measured simultaneously in a single reaction, effectively saving time and resources. Additionally, this assay can be designed to determine a variety of analytes, including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies.

The Foundation of Cytometric Bead Array (CBA)

Cytometric Bead Array (CBA) is based on the flow cytometry application. Capture beads in assay of different sizes or colors are used for multiplexed immunoassays, which have been conjugated with a specific antibody. The detection reagent provides a fluorescent signal displayed in proportional to the amount of bound analyte. When captured beads and detector reagent are incubated with an unknown sample containing target cytokine analytes, sandwich complexes (captured bead + cytokine analyte + detection reagent) are formed. These complexes can be measured by flow cytometry to identify the bead and the detector with fluorescence.

Cytokine Analysis by ELISA or CBA

Taking advantage of ELISA and CBA assay, Creative Biolabs can offer optimized cytokine analysis services for our customers, which are available to detect cytokines for several species, such as humans, rats, and mice. Meanwhile, CBA assay can also be used for the measurement of cytokines from serum and tissue culture.

Creative Biolabs is devoted to assisting clients in various cytokine analysis services, which including but not limited to ELISA and CBA. We are committed to providing one-stop cytokine release syndrome (CRS) management solutions to reducing the overall project development timeline for our clients. For further details, please directly contact us for more information.

References

Anloague, Aric, and Jonathan W. Lowery Ph D. "Comparison of Human Cytokine Array, Cytokine Bead Arrays, and Protein ELISA in determining cytokine production in media supernatant from in vitro strain of human dermal fibroblasts." Capstone Final Project (2019).
Castillo L, MacCallum DM. Cytokine measurement using cytometric bead arrays. In Host-Fungus Interactions 2012 (pp. 425-434). Humana, Totowa, NJ.
Medeiros NI, Gomes JA. Cytometric bead array (CBA) for measuring cytokine levels in chagas disease patients. In T. cruzi Infection 2019 (pp. 309-314). Humana Press, New York, NY.

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