ELISA & Cytometric Bead Array based Cytokine Analysis Service

ELISA and cytometric bead array-based cytokine analysis is indispensable for the precise quantification of soluble immune mediators, providing critical insights into inflammatory status, immune cell activation, and pathological mechanisms. Creative Biolabs' ELISA and Cytometric bead array-based Cytokine Analysis Service delivers comprehensive in vitro cytokine profiling utilizing both ELISA and cytometric bead array technologies, enabling robust and specific detection of a broad spectrum of pro- and anti-inflammatory cytokines, chemokines, and growth factors. The key advantages of our offering lie in its high sensitivity, wide dynamic range, multiplexing capability for efficient multi-analyte detection from minimal sample volumes, and rigorous quality control ensuring exceptional reproducibility and accuracy.

Introduction

ELISA and cytometric bead array represent two cornerstone technologies for the quantitative detection of cytokines secreted by immune cells. While traditional ELISA provides high-sensitivity measurement of a single analyte, bead-based cytometric bead array platforms enable the simultaneous multiplexed quantification of multiple cytokines within a single sample. These assays are indispensable for precisely characterizing the immune response, allowing researchers to decipher complex cytokine profiles and gain critical insights into immune cells activation states under both physiological and pathological conditions.

Fig.1 Array-based platforms for cytokine release quantification.¹

ELISA and Cytometric bead array-based Cytokine Analysis Service at Creative Biolabs

Creative Biolabs' ELISA and Cytometric bead array-based Cytokine Analysis Service provides optimized cytokine analysis services tailored to diverse research needs. Our platform enables precise and reliable detection of cytokines across multiple species. Furthermore, the cytometric bead array -based approach extends its utility to complex biological matrices such as serum and tissue culture supernatants, facilitating comprehensive cytokine profiling in various experimental settings. With a commitment to accuracy and reproducibility, we support drug development and immunology studies through robust, species-flexible, and matrix-adaptable analytical solutions.

What we can offer

We provide a multi-tiered technical portfolio for cytokine analysis, designed to deliver solutions ranging from robust classical quantification to high-plex multiplexing, ensuring both reliability and efficiency for diverse research needs.

Our Service Process

Required starting materials:

• Compound/Therapeutic Agent Details: Specific concentration, administration route, and proposed mechanism of action (if known).
• Sample Matrix: Detailed information on the type of biological sample and volume available.
• Target Cytokine Panel: A prioritized list of required cytokines/chemokines, or the indication area (e.g., neuroinflammation, CRS) for a consultation on panel design.

Key Steps Involved:

Final Deliverables:

• Quantitative Data Report: Normalized concentrations for all analytes across all samples, including all raw plate readouts and standard curve performance metrics.
• Scientific Interpretive Summary: A concise report highlighting statistically significant differences, biological conclusions, and correlation analysis between analytes.

Key Advantages

• Maximized Sample Economy: Guaranteed data acquisition using minimal sample volumes, maximizing the data yield from precious clinical or in vitro materials.
• Complex Sample Expertise: Optimized protocols for reliable cytokine measurement in challenging matrices, including cerebrospinal fluid (CSF), tissue lysates, and iPSC-derived cellular supernatants.
• End-to-End Customization: Expert panel design, assay development, and full validation for bespoke cytokine/chemokine targets across virtually any biological species or matrix.

FAQ

Why Choose Us?

Building upon advanced technology platforms and deep industry knowledge, we utilize methods such as ELISA and cytometric bead array for multi-cytokine quantification. Our continuous optimization and process integration directly increase the accuracy and throughput of these assays, thereby delivering robust data that supports critical research in drug development and immunology.

Customer Reviews

"Using Creative Biolabs' ELISA and Cytometric bead array-based Cytokine Analysis Service in our research has significantly improved the detection of low-level central nervous system biomarkers in CSF samples, resolving previous non-concordant data we had from alternative platforms. "— Dr. An Hg.

"The implementation of Creative Biolabs' ELISA and cytometric bead array -based cytokine analysis has streamlined our large-scale in vitro screening workflow. This approach enables us to robustly profile over 20 distinct analytes within microglial supernatants, thereby extracting maximal informational value from our precious, limited-availability iPSC-derived cellular models. "— Prof. Ka Lz.

"Employing Creative Biolabs' ELISA/cytometric bead array and IC Flow services has advanced our understanding of T cell function, successfully pinpointing the specific T helper subset responsible for IFN-γ secretion upon antigen challenge."— Dr. Js Mo.

How to contact us?

At Creative Biolabs, we offer an extensive suite of cytokine analysis services, including ELISA and cytometric bead array, coupled with end-to-end CRS management solutions. Our goal is to streamline your research and development process, saving valuable time. To discuss your specific needs, we invite you to reach out to our team.

Reference

1. Maguire, Emily et al. "Assaying Microglia Functions In Vitro." Cells vol. 11,21 3414. Distributed under Open Access License CC BY 4.0, without modification. https://doi.org/10.3390/cells11213414.