Flow Cytometry-based Expanded TIL Phenotypic Profiling Service

In the ever-evolving field of immuno-oncology, understanding the functional landscape of tumor-infiltrating lymphocytes (TILs) is critical for advancing TIL-based therapies. At Creative Biolabs, our flow cytometry-based phenotypic profiling is a robust, high-resolution technique that provides precise, quantitative data, allowing for a comprehensive view of phenotypic and functional heterogeneity within TIL populations.

Introduction to TIL Subsets and Biomarkers

Tumor-infiltrating lymphocytes (TILs) consist of a diverse subset of immune cells that penetrate tumor tissues. Each subset carries unique biomarkers that not only define their functional roles but also their interactions with tumors and other immune cells. For instance, CD3, CD4, and CD8 markers are essential for identifying T cells, while CD19 is used for B cells. The presence of regulatory T cells, often marked by CD25 and FoxP3, is of particular interest due to their immune-suppressive functions.

Fig.1 Some types of TILs and their function in cancer.¹

The heterogeneity and complexity of TILs necessitate advanced technologies to accurately characterize these cells. Flow cytometry stands out as a powerful analytical tool, capable of immunophenotyping and identifying functional states of immune cells through surface and intracellular marker detection.

Flow Cytometry Based TIL Phenotypic Profiling Services

At Creative Biolabs, we provide comprehensive TIL phenotypic profiling services that enable researchers to gain deep insights into TILs, offering precise characterization of immune cell populations within the tumor microenvironment. Our experts employ state-of-the-art flow cytometry techniques to conduct comprehensive phenotypic profiling of TILs. Our Flow cytometry stands out as a powerful tool, enabling the simultaneous analysis of multiple parameters at the single-cell level. This precision allows for an in-depth exploration of the cell surface and intracellular markers that define TIL subsets and their functional states. Utilizing advanced flow cytometry techniques, we provide detailed analyses of cell surface markers, functional responses, and immune phenotypes, empowering studies in cancer immunology and therapeutic development.

• Assessment of various TIL markers, including CD3, CD4, CD8, CD28, CD62L, PD1, FoxP3, GITR, CTLA4, etc.
• Identification of TIL status (TIL activation, TIL exhaustion)
• Identification of TIL subsets (T cells, Treg cells, memory T cells, B cells, etc.)
• Analysis of extracellular and intracellular markers, mono- and multi-parametric analysis
• Analysis of cytokine production and degranulation markers

Key Features of Our Service

Partner with us to enhance your research and unlock the potential of TIL profiling in advancing cancer treatment strategies. Please contact us to learn more about our flow cytometry solutions and how we can support your TIL therapy projects.

Reference

1. Dai, Xiaofeng, and Kaiyuan Zhu. "Cold atmospheric plasma: Novel opportunities for tumor microenvironment targeting." Cancer Medicine 12.6 (2023): 7189-7206. Distributed under Open Access license CC BY 4.0, without modification.