Expanded TIL Phenotypic Profiling Services

To support advancements in TIL therapy, Creative Biolabs offers specialized phenotypic profiling services tailored to expanded TILs. Our services provide an in-depth analysis of TIL characteristics, including immune cell subsets, activation markers, exhaustion profiles, and functional phenotypes.

Phenotypes of TILs

Tumor-infiltrating lymphocytes (TILs) are a heterogeneous group of immune cells that infiltrate tumor tissues. They play a critical role in the body's immune response to cancer. The presence and activation of TILs within the tumor environment are often indicative of the immune system's effort to combat malignancies. TILs comprise various subsets, including CD8+ cytotoxic T cells, CD4+ helper T cells, regulatory T cells (Tregs), and B cells. Key biomarkers typically employed in profiling TILs include PD-1, CTLA-4, LAG-3, and TIM-3 for T cells, reflecting their activation state and exhaustion levels. Each of these subsets has unique roles in mediating anti-tumor immunity, making their study crucial in designing effective immunotherapies.

Fig.1 The main TILs in cancer.¹

TIL Phenotypic Profiling Services

Creative Biolabs offers expansive phenotypic profiling services aimed at advancing the development of TIL-based therapies. Our phenotypic profiling services encompass a comprehensive array of analyses, including the identification of activation markers, exhaustion markers, and cytokine profiles. Understanding these parameters is critical for evaluating TIL fitness and their capacity to sustain anti-tumor responses. By leveraging our extensive expertise and state-of-the-art technologies, we provide detailed insights into TIL heterogeneity and functionality.

At Creative Biolabs, we employ advanced techniques such as flow cytometry (FC) and immunohistochemistry (IHC) to conduct thorough phenotypic profiling of TILs. We aim to provide detailed insights into the diversity, functionality, and spatial organization of TILs within the tumor milieu.

Advantages of Our Services

• Comprehensive Analysis: Our Fc and IHC approaches ensure thorough analysis of both the phenotypic makeup and spatial distribution of TILs, providing a complete picture of the tumor microenvironment.
• Expertise and Experience: With years of experience in immunological profiling and TIL research, our team offers unparalleled insights and guidance in TIL therapy development.
• Customized Solutions: We tailor our services to meet the specific needs of each client, ensuring that results are relevant and actionable for your therapeutic goals.

One-stop TIL Development Platform

At Creative Biolabs, we offer a comprehensive service package including isolation, expansion, and deep phenotypic analysis of TILs from a wide variety of tumor samples. We provide a robust platform for characterizing TIL subsets, identifying potential therapeutic targets, and enhancing the understanding of tumor immunity. Our services are tailored to meet the specific needs of your research, whether it's for basic exploration, therapeutic development, or clinical applications.

Reference

1. Bai, Ziyi, et al. "Tumor-infiltrating lymphocytes in colorectal cancer: the fundamental indication and application on immunotherapy." Frontiers in Immunology 12 (2022): 808964. Distributed under Open Access license CC BY 4.0, without modification.