CellRapeutics™ RNA Spin Kit(CART-012CL)


Product Name
CellRapeutics™ RNA Spin Kit
Cat. No.
CART-012CL
General Description
96-well format using suitable vacuum manifolds or suitable centrifuges.

or fast and efficient purification in a 96-well format
6 samples can be parallel-processed with high reproducibility in under 70 min
Integrated wash plate eliminates risk of contamination
Flexible processing by vacuum or centrifugation
DNase I treatment removes genomic DNA, leading to high-quality total RNA product CellRapeuticsTM RNA Spin Kit can be used to reproducibly isolate total RNA from cultured cells, tissue, bacteria or yeast. It allows for the simultaneous processing of 96 samples via either centrifugation or vacuum-based protocols that can easily fit onto existing automated systems. The isolated RNA is of a high enough quality and quantity (up to 100 µg) for multiple downstream experiments, including sensitive enzymatic applications like quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension, and RNase protection assays. Therefore, the RNAspin Kit is a flexible, high-throughput solution for total RNA isolation.
Applications
Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeast
Can also be used in a fully automated flow with vacuum on common laboratory workstations
Suitable for direct use in downstream applications like Quantitative Reverse Transcriptase- PCR (QRT-PCR), Primer Extension, RNase Protection Assays, Cdna Synthesis and Microarray Analysis
Preparation Note
Total Time of Preparation: 70 min/4 preps
Binding Capacity
100 µg
Sample Size [Animal Tissue (Vacuum)]
10 mg tissue-30 mg tissue
Sample Size [Animal Tissue (Centrifuge)]
30 mg tissue
Sample Size [Cell Culture Vacuum]
2 × 106 cells
Sample Size [Cell Culture Centrifuge]
1 × 107 cells
Typical Yield [Animal Tissue Vacuum]
Up to 40 µg
Typical Yield [Animal Tissue Centrifuge]
Up to 100 µg
Typical Yield [Cell Culture Vacuum]
Up to 20 µg
Typical Yield [Cell Culture Centrifuge]
Up to 100 µg
RNA Purity [A260/A280]
1.8-2.2
Product Size
Elution Volume Min.: 50 µl Elution Volume Max.: 130 µl

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-048-3343

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