Creative Biolabs offers assay services of evaluating the stability of TCR/CD3 complex to ensure the harvest of high quality TCR-modified T cells. The effective interaction of CD3 with TCR and the ξ-chain is required for the generation of activation signal in T lymphocytes. Increased stability of the TCR/CD3complex contributes to the enhanced antitumor response activity of T cells. At Creative Biolabs, the TCR/CD3 complex stability assay can be achieved via immunoprecipitation and immunoblot.
Figure: T cell receptor (TCR) complex
T-cells are a subset of lymphocytes that play a large role in the immune response targeting to various antigens. When T lymphocytes encounter antigens via the T cell antigen receptor (TCR), information about the quantity and quality of antigen engagement is relayed to the intracellular signal transduction machinery, but the TCR itself lacks a significant intracellular domain, resulting in lacking signal transduction ability. Therefore, it is usually associated with CD3 molecules which contain intracellular signaling domains to couple the TCR/CD3 complex as the downstream signaling machinery. The TCR/CD3 complex, also known as the TCR complex, is composed of six subunits which are expressed on the cell surface in a coordinate pattern after assembly in the endoplasmic reticulum (ER). The TCR/CD3 complex is assembled after a series of pairwise interactions involving the formation of dimmers of CD3 epsilon (CD3ε) with either CD3 gamma (CD3γ) or CD3 delta (CD3δ). These dimmers assemble with TCR alpha (TCR α )and TCR beta (TCRβ) chains, and finally, the CD3ξ (CD3 zeta) homodimer is added to allow export of the full complex from the ER. The main task of the TCR/CD3 complex includes two parts: ligand recognition and signal transduction from infectious agents. And it divides the tasks among its different components. In detail, the TCR contain variable regions and are responsible for antigen/MHC recognition; while the other four CD3ε, CD3γ, CD3δ and CD3ξ-chain are invariable and contain long cytoplasmic tails capable of interacting with the intracellular signal transduction machinery. Since the TCR-CD3 complex serves a critical role in the differentiation, survival, and function of T cells, and receptor triggering elicits a complex set of biological responses that serve to protect the organism from infectious agents, it is of great importance to study the TCR/CD3 complex stability of TCR-modified T cells in order to achieve an effective disease-targeting therapy.
With full understanding of the TCR/CD3 complex, Creative Biolabs is committed to offering high-standard TCR/CD3 stability assay to every client. If you have any special question about the issues of TCR products and services, please contact our scientists for help.
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