VivoCAR™ Anti-KLK3 (5A10) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX132)

Name: VivoCAR™ Anti-KLK3 (5A10) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX132
Rating: 5 (1 reviews)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-KLK3 (5A10) h(CD28-41BB-CD3z) CAR circRNA utilizes the 5A10 clone to target the PSA protein re-expressed on the surface of prostate cancer cells. This product is significant for its ability to recognize PSA-positive malignant cells in the circulation and bone marrow. By delivering the CAR via the circRNA platform, we enable a transient therapeutic surge of CAR-T activity. The non-integrating nature of the transcript ensures that the therapy naturally tapers off, providing a safe and repeatable method for the systemic clearance of metastatic prostate cancer without long-term genetic risk.

Add to Cart

Target Information

Target
KLK3

Common Name
KLK3

Alternative Names
APS, KLK2A1, PSA, hK3

Full Name
Kallikrein-Related Peptidase 3

Introduction
KLK3, universally known as Prostate-Specific Antigen (PSA), is a serine protease produced by the epithelial cells of the prostate gland. While largely a secreted protein used as a serum biomarker for prostate cancer, a portion of PSA remains associated with the cell membrane or localized within the tumor microenvironment of malignant prostatic tissue. CAR-T cells targeting KLK3 are designed to recognize these tumor-associated forms, providing a highly specific pathway for treating advanced prostate malignancies.

Targeting Diseases
Prostate Cancer, Metastatic Castration-Resistant Prostate Cancer (mCRPC)

UniProt ID
P07288

Gene ID
354

Target Species
Human

Antibody Clone
5A10

scFv-Host Species
Human

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Customer Reviews and Q&As

There are currently no customer reviews or questions for VivoCAR™ Anti-KLK3 (5A10) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX132). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.