VivoCAR™ Anti-MICA (8C7) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX49)

Name: VivoCAR™ Anti-MICA (8C7) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX49
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-MICA (8C7) h(CD28-41BB-CD3z) CAR circRNA features the 8C7 clone, specifically optimized for its ability to block MICA shedding-a major mechanism of immune evasion. The importance of the 8C7 clone for in vivo CAR-T therapy lies in its dual action: direct cytolytic targeting and the preservation of membrane-bound MICA. The circular RNA architecture ensures a stable, high-level translation of the CAR, facilitating a potent therapeutic window that empowers the host's lymphocytes to overcome the soluble-ligand "smoke screen" used by metastatic tumors to disable immune surveillance.

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Target Information

Target
MICA

Common Name
MICA

Alternative Names
MIC-A, PERB11.1

Full Name
MHC Class I Polypeptide-Related Sequence A

Introduction
MICA is a stress-induced surface molecule expressed on epithelial and endothelial cells in response to cellular stress, infection, or malignant transformation. It is a ligand for the activating NKG2D receptor on NK cells and CD8+ T cells. Tumor cells often upregulate MICA but then shed it to evade immune surveillance. CAR-T cells targeting MICA can be used to directly kill MICA-expressing tumors or to target the soluble form of MICA to enhance NK cell activity.

Targeting Diseases
Various Solid Tumors (e.g., Colorectal, Ovarian, Prostate Cancer), Melanoma

UniProt ID
Q29983

Gene ID
100507436

Target Species
Human

Antibody Clone
8C7

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

VivoCAR™ Anti-MICA (8C7) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX49)

Target Information

Customer Reviews and Q&As

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