Anti-Human CD30 (Brentuximab) h(CD28-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF79)

Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-CD30 (Brentuximab) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human CD30. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-CD30 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-CD30 (Brentuximab) linked to 2nd generation CAR containing CD28 and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

Target
CD30

Components
This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
1. A CAR lentiviral vector
• Anti-CD30 (Brentuximab) h(CD28-CD3ζ) CAR
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. CAR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

CAR Generation
2nd-generation CAR

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-CD3ζ

Target

Gene Name
TNFRSF8

Full Name
TNF receptor superfamily member 8

scFv
Brentuximab

Host Species
Human

Target Species
Human

Gene ID
943

Uniprot ID
P28908

Target Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Synonyms
CD30; Ki-1; D1S166E; TNFRSF8; TNF receptor superfamily member 8

Related Disease
Hodgkin lymphoma

Related Resources

Overview of CD30-Targeted CAR Cell Therapies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.