Natural Killer Cell based Artificial T Cell Stimulator Development Service

Artificial T Cell Stimulators Improve NK Cell-based Immunotherapies

Artificial T cell stimulators represent a significant advancement in NK cell immunotherapy by providing a controlled and robust platform for NK cell expansion and activation. These stimulators effectively mimic the interactions between T cells and antigen-presenting cells, driving robust NK cell proliferation while maintaining their cytotoxic function, which enables the generation of large numbers of potent NK cells from limited sources and allows for precise control over the activation and differentiation of these cells. The versatility of artificial T cell stimulators, combined with their potential for clinical translation, makes them a promising strategy for developing more effective and personalized NK cell-based therapies against a range of malignancies.

Fig.1 Cancer immunity cycles depend on T cells, NK cells, and TAMs.^1,3

Natural Killer Cell based Artificial T Cell Stimulator Development Service at Creative Biolabs

Creative Biolabs provides a specialized NK cell-based artificial T cell stimulator development service to empower NK cell-based immunotherapies. Our expertise in immunology and cell biology enables us to design and engineer artificial T cell stimulators to precisely mimic natural antigen-presenting cells, achieving homogeneous NK cell activation through tailored delivery of specific activation signals. At the same time, we provide end-to-end support, from initial design and engineering to rigorous functional validation, ensuring efficient and high-quality outcomes. Our flexible approach allows for customized stimulator designs and precise control over stimulation parameters, accelerating scientific progress and facilitating the rapid development of novel NK cell-based immunotherapies for our clients.

Featured Advantages

• Homogeneous NK cell activation
• Customized design and flexibility
• Precisely control NK cell activation
• Rapid delivery of results
• High-quality and efficient service

Case Study

Background: NK cells are crucial for anti-tumor immunity, including against multiple myeloma (MM). Umbilical cord blood (CB) is a valuable source of NK cells for allogeneic therapies. However, the effective clinical application requires a significant expansion of CB-derived NK (CB-NK) cells.

Method: This study developed a novel method for expanding CB-NK cells using artificial stimulators in a gas-permeable culture system. CB units, both fresh and cryopreserved, were utilized. NK cell expansion was assessed over 14 days, and the purity, phenotype, and cytotoxicity of the expanded CB-NK cells were thoroughly characterized.

Results: The artificial stimulator-based method achieved substantial NK cell expansion, with an average fold increase of 1848 from fresh and 2389 from cryopreserved CB. Expanded CB-NK cells exhibited high purity (>95% CD56+/CD3−) and maintained a functional phenotype with preserved cytotoxicity against various MM targets. Importantly, artificial stimulator-expanded CB-NK cells demonstrated significant in vivo anti-MM activity in a xenograft mouse model.

Fig.2 CB-NK cell phenotype in response to artificial stimulants.^2,4

Fig.3 CB-NK cells generated by artificial stimulators create immunological synapses with myeloma targets and are cytotoxic to them.^2,4

Associated Services

To support a variety of immunological research applications, Creative Biolabs also provides a comprehensive range of specialized artificial T cell stimulator development services for other subtypes, which include:

• Antigen-Specific CD8+ T Cell based Artificial T Cell Stimulator Development Service
• Antigen-Specific CD4+ T Cell based Artificial T Cell Stimulator Development Service
• Polyclonal CD3+ T Cell based Artificial T Cell Stimulator Development Service
• Chimeric Antigen Receptor Transduced T Cell based Artificial T Cell Stimulator Development Service
• Invariant Natural Killer T Cell based Artificial T Cell Stimulator Development Service

Please get in touch with us and discuss your individual project needs if you would like to discuss the creation of a flexible, bespoke artificial T cell stimulator designed for NK cell stimulation.

References

1. Yang, Ya-long, et al. "T cells, NK cells, and tumor-associated macrophages in cancer immunotherapy and the current state of the art of drug delivery systems." Frontiers in Immunology 14 (2023): 1199173.
2. Shah, Nina, et al. "Antigen presenting cell-mediated expansion of human umbilical cord blood yields log-scale expansion of natural killer cells with anti-myeloma activity." PloS one 8.10 (2013): e76781.
3. Distributed under Open Access License CC BY 4.0, without modification.
4. Distributed under Open Access License CC BY, without modification.