Creative Biolabs offers various strategies to perform the transfer of TCR genes into primary T cells. Transferring an exogenous gene into T cells is a barrier impedes the development of TCR-modified T cells immunotherapy. However, with our up-to-date equipments, experienced project teams, we can develop the most appropriate strategy to achieve the effective gene transduction into T cells.
Gene transfer technologies offer the ability to genetically modify the cells. Generally, to choose the appropriate method of DNA transfer mainly depends on the target cells and the objectives of gene manipulation. There are two classes of approaches that can be used for DNA transfer. The first strategy utilizes natural methods to generate gene transfer, this class of methods includes conjugation, bacterial transformation, phage transduction, viral transduction, transposition and agrobacterium mediated transfer. DNA transfer can also be realized by artificial methods, which contains physical methods (macroinjection, microinjection, protoplast fusion and biolistics transformation), chemical methods (calcium phosphate, polyethene glycol, DEAD-Dextran and liposome) and electrical methods (electroporation and electrofusion). All these methods are powerful and ideal tools for scientific and clinical researches and have possible applications in gene therapy. In the development of TCR-modified T cells immunotherapy, the gene delivery is often done by treating cells with viral vectors such as retrovirus vectors and lentiviral vectors, or electroporation. The genome of virus has been manipulated to carry exogenous DNA. With engineering development, the immunogenicity of adenovirus vectors has been reduces which enhance their applications for long-term gene transfer in different tissues. Viral vectors used in scientific and clinical researches for gene therapy include oncoretroviruses (e.g. moloney murine leukaemia virus), lentiviruses, retroviruses, adenoviruses, adeno-associated viruses and herpes simplex virus-1. Using the method of viral vector-based gene transfer can increase the stability of integration of the viral genome into the host. Electroporation employs high-voltage electrical pulse to introduce DNA into cells.
Based on the type of transduced immune cells and the specific requirements of every client, scientists from Creative Biolabs will design the appropriate strategy to integrate modified TCR into the host cells.
The most popular strategies used for TCR gene transfer:
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