Glucose Uptake Quantification Service

The Importance of Glucose Metabolism for CD8⁺ T Cell Function

Naive CD8⁺ T cells fundamentally depend on mitochondrial oxidative phosphorylation (OXPHOS) for their ATP generation. Meanwhile, IL-7 signaling preserves quiescent T cell survival through STAT5-mediated AKT activation, which drives GLUT1 upregulation and subsequent glucose import to maintain OXPHOS activity. The therapeutic challenge arises from tumor biology - malignant cells dramatically accelerate glycolysis, effectively starving the tumor microenvironment (TME) of glucose. This metabolic competition critically compromises T cell functionality while fueling tumor advancement. These mechanistic insights position glucose metabolism modulation as a strategic frontier in oncology treatment development.

Fig.1 Auto-glycolysis levels following CD8⁺ T cell activation.¹

Glucose Uptake Quantification Service at Creative Biolabs

Creative Biolabs delivers a highly accurate and cost-efficient glucose uptake analysis service, specifically designed to map CD8⁺ T cell metabolic behavior in both normal and pathological states. Our platform combines proven biochemical techniques with advanced detection technologies to measure glucose consumption patterns in purified CD8⁺ T cell populations. The core methodology employs fluorescent glucose tracers like 2-NBDG, allowing real-time cellular uptake monitoring through flow cytometry or fluorescence microscopy. For enhanced sensitivity requirements, we implement radiolabeled glucose derivatives analyzed via precision scintillation counters. These complementary strategies generate detailed metabolic fingerprints of T cell populations - essential intelligence for decoding their functional plasticity in disease microenvironments and advancing next-generation immunotherapies.

Crucially, our operational framework integrates rigorous quality assurance measures throughout the testing pipeline. Standardized protocols combined with regularly calibrated instrumentation ensure minimal batch-to-batch variation and superior data reproducibility. This operational synergy enables researchers to accelerate therapeutic discovery cycles while maintaining rigorous evidentiary standards for regulatory submissions.

Suitable Samples

Our service is flexible and suitable for various samples, which include:

• Cultured cells
• Tissue samples
• Blood samples
• Primary cells

Key Features

Associated Services

In addition to the glucose uptake measurement service, we also provide a series of services to characterize the amino acid metabolism of CD8⁺ T cells in specific environments, the impact of hypoxic for CD8⁺ T cell function, acidity assessment, key cytokine expression, etc. furthermore, we offer in-depth metabolite analysis of both physiological and TME CD8⁺ T cells, providing not only a detailed compositional breakdown of cellular metabolites but also rigorous evaluation of the intervention effects of specific targeted compounds, enabling a deeper understanding of metabolic pathways and potential therapeutic modulation.

• Physiological CD8⁺ T Cell Metabolic Profiling Service
• Tumor Microenvironment (TME) CD8⁺ T Cell Metabolic Profiling Service
• Amino Acid Utilization Analysis Service
• Tumor Microenvironment (TME) Hypoxia and Acidity Assessment Service
• Comprehensive Metabolite Profiling Service
• Key Cytokine Expression Analysis Service

Creative Biolabs stands as a leading force in T cell research, backed by years of hands-on experience and robust scientific infrastructure. Our team delivers precision-crafted services spanning comprehensive metabolic analysis, precise evaluation of glucose transport mechanisms, and in-depth exploration of nutrient-signaling pathways. Through customized approaches and rigorous quality control, we consistently deliver trustworthy, repeatable results that advance CD8⁺ T cell investigations. Please feel free to reach out to us with your meaningful projects.

Reference

1. Cao, Jiaying, et al. "Effects of altered glycolysis levels on CD8⁺ T cell activation and function." Cell Death & Disease 14.7 (2023): 407. Distributed under Open Access License CC BY 4.0, without modification.