TME CD8⁺ T Cell Metabolic Profiling Service

CD8⁺ T Cell Metabolism in TME

In the tumor microenvironment, CD8⁺ T cells play a critical role in attacking cancer, but their success largely depends on metabolic dynamics. When CD8⁺ T effector cells are active, they depend on glycolysis to fuel their tumor-killing functions. If this energy pathway falters, so does their ability to eliminate cancer cells. On the other hand, tumor cells often flood the environment with lactate, a glycolysis byproduct. Excess lactate buildup can block CD8⁺ T cells from exporting it, stifling their growth and weakening their cancer-fighting power. This metabolic battle—where nutrients are fiercely competed over and toxic metabolites like lactate pile up—creates a harsh landscape that cripples CD8⁺ T cells' ability to effectively combat tumors.

Fig.1 The metabolic interaction between tumor cells and T effector cells.^1,3

TME CD8⁺ T Cell Metabolic Profiling Service at Creative Biolabs

To comprehensively identify CD8⁺ T cell metabolism in TME, Creative Biolabs delivers a cost-effective TME CD8⁺ T cell metabolic profiling service to assist global customers in therapeutic target discovery and targeted therapeutics development.

In particular, our service provides a comprehensive analysis of CD8⁺ T cells within the TME to uncover key insights for therapeutic development. We begin by isolating and enriching CD8⁺ T cells from tumor tissue. Next, we delve into the metabolic landscape of these cells to quantify critical processes and intracellular metabolite levels. Meanwhile, we examine the genetic underpinnings of these metabolic states and immune responses. Specifically, we evaluate gene expression profiling to reveal the expression of metabolic pathway genes and immune checkpoint-related genes while assessing cytokine production, cytotoxicity, and proliferation to understand the functional implications. Altogether, our integrated profiling enables customers to identify key signatures and potential therapeutic targets, ultimately promoting targeted immunotherapeutics discovery and development.

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Moving beyond standard TME CD8⁺ T cell metabolic profiling, our services explore the intricate interplay of metabolic rewiring in these immune effectors. We deliver comprehensive characterization of physiological metabolic adaptations triggered by diverse stimuli, shedding light on their adaptation mechanisms across diverse physiological contexts. Our customized analytical framework incorporates specialized metrics—from real-time amino acid utilization rates to glucose transporter dynamics—that pinpoint nutrient dependencies and expose metabolic liabilities governing CD8⁺ T cell efficacy. This granular, pathway-centric approach empowers researchers to map rate-limiting metabolic nodes and engineer precision interventions for fine-tuning T cell behavior in both homeostasis and disease pathogenesis.

• Physiological CD8⁺ T Cell Metabolic Profiling Service
• Glucose Uptake Quantification Service
• Amino Acid Utilization Analysis Service
• Tumor Microenvironment (TME) Hypoxia and Acidity Assessment Service
• Comprehensive Metabolite Profiling Service
• Key Cytokine Expression Analysis Service

If you are looking for a reliable company to characterize interested TME CD8⁺ T cells and evaluate the effectiveness of developed therapeutics, please don't hesitate to get in touch with us.

Reference

1. Ping, Yu, et al. "Reprogramming T-Cell metabolism for better anti-tumor immunity." Cells 11.19 (2022): 3103. Distributed under Open Access License CC BY 4.0, without modification.