Physiological CD8⁺ T Cell Metabolic Profiling Service

The Necessity of CD8⁺ T Cell Metabolism Profiling

Metabolic regulation serves as the master switch governing CD8⁺ T cell capabilities - their capacity to fight pathogens and cancerous growths, while maintaining immunological memory. When antigen recognition occurs, these immune soldiers transition through immediate metabolic rewiring to fuel their explosive replication and transformation into specialized effector cells or long-lived memory reserves. Yet the precise biochemical pathways directing CD8⁺ T cell fate determination remain incompletely mapped, like uncharted metabolic circuitry controlling cellular destiny. This incomplete understanding underscores why decoding these metabolic requirements represents the holy grail for designing potent immunotherapeutic strategies that could amplify our body's natural defense mechanisms.

Fig.1 The antitumor-immune response and T-cell fate and function are both influenced by metabolic reprogramming.^1,3

Physiological CD8⁺ T Cell Metabolic Profiling Service at Creative Biolabs

At Creative Biolabs, we offer a comprehensive and affordable service for physiological CD8⁺ T cell metabolic profiling, enabling researchers to investigate how metabolic changes impact CD8⁺ T cell behavior in various physiological and pathological states, ultimately aiding in the design of targeted immunotherapeutic strategies.

To accurately profile CD8⁺ T cell metabolism in a physiological context, we employ a comprehensive strategy that begins with ex vivo isolation of primary cells from biological samples, followed by stimulation using physiologically relevant cues like peptide-MHC tetramers and antigen-presenting cells. We then leverage advanced metabolic assays, such as flux cytometry and targeted metabolomics, to quantify key metabolic pathways. To contextualize these findings, we utilize spatial metabolomics and imaging to assess metabolic interactions within the tissue microenvironment and integrate these metabolic profiles with functional assays to correlate metabolism with effector functions. Finally, systems biology approaches are used to integrate multi-omics data, creating robust models that identify critical regulatory nodes and provide a holistic understanding of CD8⁺ T cell metabolism.

Throughout the whole process, we implement strict quality control and adhere to regulatory development. Combined with the proficiency of our experienced research team, this commitment to excellence enables us to deliver accurate results rapidly and efficiently.

Attractive Advantages

• Physiological Relevance---accurately mimics in vivo conditions
• Multi-Omics Integration---a combination of various advanced techniques
• Robust Data Quality---ensured data reliability and reproducibility
• Rapid Result Delivery---efficient data acquisition and timely results
• Therapeutic Applications---support targeted immunotherapies development

Data Supporting

Summary: This study employed a comprehensive metabolomics approach, combining multiple flow injection analysis (FIA) and liquid chromatography (LC) methods with high-resolution mass spectrometry (MS), to meticulously track the metabolic reprogramming of CD8⁺ T cells during their transition from a naïve to effector state. Analyzing nine time points, the researchers identified significant time-dependent changes in a vast array of metabolic features, particularly within the first 48 hours.

Results: Key findings included the dynamic remodeling of the polyamine biosynthesis pathway and a dramatic shift in phosphatidylcholine acyl chain composition, marked by a depletion of polyunsaturated species, especially those containing 20:4 fatty acid. This extensive dataset, encompassing over 11,000 features, provides a valuable resource for future immunometabolism research.

Fig.2 Key metabolite time courses in the production of polyamines.^2,3

Associated Services

In addition, we extend our capabilities to offer specialized services tailored to the unique microenvironment of tumor-infiltrating lymphocytes (TME). This includes detailed characterization of TME CD8⁺ T cell metabolism, providing insights into the metabolic adaptations within the tumor context. Furthermore, we provide customized assessments, such as precise quantification of amino acid utilization and glucose uptake, allowing for a granular understanding of nutrient dependencies and metabolic vulnerabilities on CD8⁺ T cell functions.

• Tumor Microenvironment (TME) CD8⁺ T Cell Metabolic Profiling Service
• Glucose Uptake Quantification Service
• Amino Acid Utilization Analysis Service
• Tumor Microenvironment (TME) Hypoxia and Acidity Assessment Service
• Comprehensive Metabolite Profiling Service
• Key Cytokine Expression Analysis Service

For inquiries regarding our specialized physiological CD8⁺ T cell metabolic profiling services, please contact us to discuss your project needs.

References

1. Kouidhi, Soumaya, Amel Benammar Elgaaied, and Salem Chouaib. "Impact of metabolism on T-cell differentiation and function and cross talk with tumor microenvironment." Frontiers in Immunology 8 (2017): 270.
2. Ping, Yu, et al. "Reprogramming T-Cell metabolism for better anti-tumor immunity." Cells 11.19 (2022): 3103.
3. Distributed under Open Access License CC BY 4.0, without modification.