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Herpes Simplex Virus (HSV) Reactivation Assay Service

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Are you currently facing challenges in evaluating antiviral efficacy against latent HSV, particularly in the context of immunosuppressive therapies like CAR T-cell treatment, or difficulty in assessing reactivation mechanisms? Creative Biolabs' HSV reactivation assay service helps you accelerate antiviral drug discovery, accurately evaluate drug candidates, and understand HSV latency and reactivation through advanced cell-based assay platforms and robust viral detection methods.

Herpes Simplex Virus Threatens Post-CART Therapy Patients

Herpes Simplex Virus (HSV) establishes lifelong latency, causing recurrent disease that current antivirals cannot eliminate. This presents a significant challenge, particularly for patients undergoing advanced immunosuppressive treatments like CAR T-cell therapy. Such therapies create a profound immunosuppressive microenvironment, making patients highly susceptible to opportunistic infections, including common and serious HSV reactivation. Viral infections, including HSV, significantly contribute to morbidity and mortality in immunocompromised individuals. The persistent clinical burden and the critical need for improved patient safety necessitate advanced HSV reactivation assays. These assays are vital for identifying novel therapeutic targets and evaluating drug candidates to prevent or reduce reactivation, ultimately improving patient outcomes post-CAR T-cell therapy.

Fig.1 Herpes simplex virus (HSV) entry and release at the cell membrane. (OA Literature) Fig.1 The process of HSV entry and release.¹

HSV Reactivation Assay Service at Creative Biolabs

Creative Biolabs' HSV reactivation assay service provides comprehensive solutions for evaluating compounds and understanding the mechanisms governing HSV latency and reactivation. Our service is designed to deliver precise, reliable data that accelerates your antiviral drug discovery and development efforts. You can expect detailed insights into compound efficacy against latent virus, identification of molecules capable of inhibiting reactivation, and a clearer understanding of viral pathogenesis, crucial for managing infections in immunosuppressed patients.

Workflow

Step 1

Cell Model Preparation

We establish and optimize appropriate in vitro latency models, typically using neuronal or neuronal-like cell lines (e.g., human neuroblastoma cells, primary sensory neurons) that faithfully recapitulate HSV latency. This involves culturing and preparing cells under conditions conducive to establishing a quiescent viral state.

Step 2

Latency Induction

Cells are infected with HSV at a low multiplicity of infection (MOI) and maintained under specific conditions (e.g., low temperature, chemical inhibitors, or neuronal co-culture) to promote the establishment of a latent infection, where viral gene expression is minimal and no productive replication occurs.

Step 3

Reactivation Induction

Latent HSV is induced to reactivate using various physiological or chemical stimuli known to trigger reactivation in vivo (e.g., stress hormones, UV irradiation, histone deacetylase inhibitors, or specific cytokines). This step ensures the model accurately reflects in vivo reactivation triggers, including those relevant to immunosuppression.

Step 4

Antiviral Treatment & Evaluation

Raw data undergoes thorough statistical analysis, data visualization, and comparison against established controls and standards. Our bioinformaticians and virologists interpret the results in the context of your project goals.

Step 5

Viral Load Quantification & Detection

Post-reactivation, we quantify viral replication and gene expression using highly sensitive methods such as quantitative PCR (qPCR) for viral genome copies, reverse transcription qPCR (RT-qPCR) for lytic gene expression, and immunofluorescence or Western blot for viral protein detection. This provides a comprehensive picture of reactivation levels.

Step 6

Data Analysis & Interpretation

The collected data undergoes rigorous statistical analysis. We provide detailed reports, including dose-response curves, statistical significance of compound effects, and comparative analyses against controls. Our expert scientists interpret the findings to provide actionable insights for your project, including implications for patient management in immunosuppressive contexts.

Our Service Advantages

High Sensitivity: Detects even subtle changes in viral reactivation.
Physiologically Relevant Models: Utilizes in vitro models that closely mimic in vivo latency, including conditions relevant to immunosuppression.
Customizable Assays: Tailored to your specific research needs and compound characteristics.
Comprehensive Data: Provides quantitative and qualitative insights into compound efficacy.
Accelerated Drug Discovery: Efficiently screens and validates potential antiviral candidates for various clinical scenarios, including post-CAR T-cell therapy.

Customer Reviews

Review One----Enhanced Efficacy Screening

Using Creative Biolabs' HSV Reactivation Assay Service in our research has significantly improved our ability to screen compounds for anti-reactivation potential, providing clearer efficacy profiles compared to standard antiviral assays. The detailed data allowed us to prioritize our lead candidates more effectively, especially for our projects targeting viral control in immunosuppressed patients.

----2024-03-15, Dr. J***s M

Review Two----Streamlined Compound Evaluation

The comprehensive workflow and clear deliverables from Creative Biolabs' HSV Reactivation Assay Service streamlined our compound evaluation process. We gained critical insights into the anti-reactivation activity of our novel molecules, which has been instrumental in guiding our preclinical development for preventing post-transplant viral complications. Their team's scientific support was exceptional.

----2025-01-10, Dr. A***a P

FAQs

Q1: What types of HSV strains can be tested with this assay?

A1: Our HSV Reactivation Assay Service is highly versatile and can accommodate a wide range of HSV-1 and HSV-2 strains, including common laboratory strains, drug-resistant variants, and specific clinical isolates. We work closely with you to select the most appropriate strain for your research objectives.

Q2: Can this service be used to evaluate both small molecules and biologics?

A2: Yes, our assay platform is adaptable for evaluating various types of compounds, including small molecules, peptides, antibodies, and other biologics. We can optimize the assay conditions to suit the specific characteristics of your test compounds, making it suitable for a broad range of antiviral development efforts.

Q3: What materials do I need to provide to start a project?

A3: To initiate your project, we typically require specific HSV-1 or HSV-2 viral strains (e.g., clinical isolates), your antiviral drug candidates or compounds for screening, and any relevant cell lines that mimic neuronal latency or are critical to your research.

Related Services

Following CAR-T cell treatment, individuals may be susceptible to infections other than HSV. We provide comprehensive infectious disease tests that target a diverse range of organisms that represent a danger to immunocompromised individuals. Our services include:

Contact Us

At Creative Biolabs, we are dedicated to advancing antiviral research through our cutting-edge HSV Reactivation Assay Service. Our expertise and comprehensive solutions are designed to accelerate your projects and bring effective antiviral therapies closer to patients, especially those in vulnerable, immunosuppressed states following treatments like CAR T-cell therapy. If you want to learn more about this service, please feel free to reach out to us.

Reference

Banerjee, Anwesha et al. "Herpes Simplex Virus: The Hostile Guest That Takes Over Your Home." Frontiers in microbiology vol. 11 733. 7 May. 2020, doi:10.3389/fmicb.2020.00733. Distributed under Open Access License CC BY 4.0, without modification.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.