VivoCAR™ Anti-ALPPL2 (h1E8) h(CD28-41BB-CD3z) CAR saRNA (XS-0326-XU85)

Name: VivoCAR™ Anti-ALPPL2 (h1E8) h(CD28-41BB-CD3z) CAR saRNA
SKU: XS-0326-XU85
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-ALPPL2 (h1E8) h(CD28-41BB-CD3z) CAR saRNA utilizes a high-efficiency saRNA backbone to drive the humanized 1E8 clone. In vivo, h1E8 is critical for targeting ALPPL2 on mesothelioma and testicular cancers. The saRNA platform achieves a biological magnification effect, ensuring robust CAR expression from a low dose. This enables T cells to reprogram in situ and target the tumor-specific ALPPL2 antigen while maintaining a dose-sparing approach for improved safety in systemic administration.

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CAR Information

Target
ALPPL2

Target Species
Human

Antibody Clone
h1E8

scFv Host Species
Human

Receptor Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation CD28-41BB-CD3ζ architecture employs a sequential costimulatory strategy, where the CD28 module drives rapid initial activation and metabolic reprogramming, while the 4-1BB component sustains long-term cellular viability and mitigates exhaustion. This tandem design, anchored by the CD3ζ signaling chain, synergistically amplifies downstream signal transduction, resulting in a balanced T-cell phenotype with robust effector capacity and prolonged durability. The integrated signaling cascade thus optimizes both kinetic expansion and persistence, enhancing antitumor efficacy.

CAR saRNA Properties

Structure
Linear

Cap
Cap 1

PolyA
Segmented PolyA

Product Type
CAR IVT saRNA

Concentration
Lot specific

Buffer
1 mM Sodium Citrate, pH 6.4 (or specialized RNA storage buffer, RNase-free).

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

saRNA Quality Control

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Target Information

Full Name
Alkaline Phosphatase, Placental-Like 2

Alternative Names
GCAP; ALPPL; ALPG

Introduction
ALPPL2 is an oncofetal protein belonging to the alkaline phosphatase family. It is highly specific to certain cancers, particularly germ cell tumors and mesothelioma, with minimal expression in normal adult tissues.

Targeting Diseases
Ovarian Cancer, Testicular Germ Cell Tumors, Lung Cancer (Adenocarcinoma), Bladder Cancer

UniProt ID
P10696

Gene ID
251

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.