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A uniCAR [Anti-PNE(GCN4) scFv-28ζ], with a CAR Adaptor [Anti-CD19 Antibody (47G4), PNE(GCN4) Tagged], a Anti-PNE(GCN4) CAR System (CAR-NR-ZP1993)

Creative Biolabs has developed a new platform of switchable CAR by using a peptide neo-epitope (PNE) in the switch molecule. In this platform, the switch molecule contains a peptide of 14-aa residues. This 14-aa PNE tag can be specifically recognized by the signaling CAR containing a PNE-specific scFv . This 14-aa PNE is found in the yeast transcription factor GCN4 and was genetically inserted at the defined site of a tumor antigen targeted scFv. The 14-aa PNE of yeast GCN4 does not exist in the human body.

Creative Biolabs provides customized Anti-PNE(GCN4) CAR design and construction services. Please contact us for more detailed information.

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CAR Schemeitc

UniCAR

  • CAR Construction
  • Anti-PNE(GCN4) scFv-CD28-CD3ζ
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and CD28. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Targeting Diseases
  • Chronic lymphocytic leukemia (CLL)

CAR Adaptor

  • Adaptor Target
  • CD19
  • Common Name
  • CD19
  • Adaptor Type
  • Fab Antibody
  • Antibody Host
  • Human
  • Antibody Clone
  • 47G4
  • CAR Adaptor Tag
  • PNE(GCN4)

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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