VivoCAR™ Anti-5T4 (A1) h(CD28-41BB-CD3z) CAR saRNA (XS-0326-XU4)

Name: VivoCAR™ Anti-5T4 (A1) h(CD28-41BB-CD3z) CAR saRNA
SKU: XS-0326-XU4
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-5T4 (A1) h(CD28-41BB-CD3z) CAR saRNA leverages a self-amplifying RNA platform to express the A1 clone, targeting the oncofetal antigen 5T4. In the context of in vivo CAR-T, the A1 clone is essential for its ability to recognize 5T4 in highly hypoxic tumor microenvironments. The saRNA-driven biological magnification ensures that even with a limited systemic dose, T cells reach the requisite expression threshold to penetrate and eliminate solid tumors while maintaining a favorable safety profile.

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CAR Information

Target
5T4

Target Species
Human

Antibody Clone
A1

scFv Host Species
Mouse

Receptor Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation CD28-41BB-CD3ζ architecture employs a sequential costimulatory strategy, where the CD28 module drives rapid initial activation and metabolic reprogramming, while the 4-1BB component sustains long-term cellular viability and mitigates exhaustion. This tandem design, anchored by the CD3ζ signaling chain, synergistically amplifies downstream signal transduction, resulting in a balanced T-cell phenotype with robust effector capacity and prolonged durability. The integrated signaling cascade thus optimizes both kinetic expansion and persistence, enhancing antitumor efficacy.

CAR saRNA Properties

Structure
Linear

Cap
Cap 1

PolyA
Segmented PolyA

Product Type
CAR IVT saRNA

Concentration
Lot specific

Buffer
1 mM Sodium Citrate, pH 6.4 (or specialized RNA storage buffer, RNase-free).

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

saRNA Quality Control

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Target Information

Full Name
Trophoblast Glycoprotein

Alternative Names
TPBG; M6P1; 5T4AG; WAIF1

Introduction
5T4 is a 72 kDa oncofetal glycoprotein characterized by leucine-rich repeats. It is highly expressed on the surface of various carcinomas but has very limited distribution in normal adult tissues. 5T4 is involved in cell adhesion, motility, and the Wnt signaling pathway, and its expression is often associated with increased tumor invasiveness and poor clinical prognosis, making it an ideal target for solid tumor CAR-T/NK therapies.

Targeting Diseases
Colorectal Cancer, Renal Cell Carcinoma, Gastric Cancer, Ovarian Cancer, Non-Small Cell Lung Cancer (NSCLC)

UniProt ID
Q13641

Gene ID
7162

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.