VivoCAR™ Anti-AFP (A18) h(CD28-41BB-CD3z) CAR saRNA (XS-0326-XU82)

Name: VivoCAR™ Anti-AFP (A18) h(CD28-41BB-CD3z) CAR saRNA
SKU: XS-0326-XU82
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-AFP (A18) h(CD28-41BB-CD3ζ) CAR saRNA employs a high-efficiency self-amplifying RNA (saRNA) backbone to express the A18 single-domain antibody. This antibody fragment is particularly valuable for in vivo applications due to its specific recognition of intracellular alpha-fetoprotein (AFP)-derived peptide fragments presented by MHC class I molecules on hepatocellular carcinoma cells. The inherent amplification property of the saRNA platform generates a potent biological signal, ensuring strong and sustained CAR expression directly at the site of T-cell engagement. This strategy enables precise targeting and elimination of AFP-presenting tumors, while the dose-sparing profile of saRNA administration helps minimize the risk of systemic toxicity, a critical consideration for patients with compromised liver function.

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CAR Information

Target
AFP

Target Species
Human

Antibody Clone
A18

scFv Host Species
Lama pacos

Receptor Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation CD28-41BB-CD3ζ architecture employs a sequential costimulatory strategy, where the CD28 module drives rapid initial activation and metabolic reprogramming, while the 4-1BB component sustains long-term cellular viability and mitigates exhaustion. This tandem design, anchored by the CD3ζ signaling chain, synergistically amplifies downstream signal transduction, resulting in a balanced T-cell phenotype with robust effector capacity and prolonged durability. The integrated signaling cascade thus optimizes both kinetic expansion and persistence, enhancing antitumor efficacy.

CAR saRNA Properties

Structure
Linear

Cap
Cap 1

PolyA
Segmented PolyA

Product Type
CAR IVT saRNA

Concentration
Lot specific

Buffer
1 mM Sodium Citrate, pH 6.4 (or specialized RNA storage buffer, RNase-free).

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

saRNA Quality Control

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Target Information

Full Name
Alpha-Fetoprotein

Alternative Names
AFPD; FETA; HPAFP

Introduction
AFP is a glycoprotein normally produced during fetal development. In adults, its presence is a major biomarker for hepatocellular carcinoma and certain germ cell tumors, where it is often presented as peptide-MHC complexes.

Targeting Diseases
Hepatocellular Carcinoma (HCC), Testicular Cancer (Non-seminomatous germ cell tumors), Ovarian Cancer

UniProt ID
P02771

Gene ID
174

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.