VivoCAR™ Anti-CD3D (F2B) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX66)

Name: VivoCAR™ Anti-CD3D (F2B) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX66
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-CD3D (F2B) h(CD28-41BB-CD3z) CAR circRNA introduces a pioneering approach to treating T-cell malignancies by targeting the CD3 delta chain directly in vivo. The F2B clone is specifically engineered to identify malignant T-cell clones while being delivered via circRNA-LNPs to healthy effector cells. This product is indispensable for research into overcoming CAR-T fratricide; the transient nature of circRNA ensures that the anti-CD3D activity is self-limiting, allowing for a strategic "hit-and-run" depletion of cancerous T-cell lineages without causing permanent, irreversible ablation of the host's entire cellular immune system.

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Target Information

Target
CD3D

Common Name
CD3D

Alternative Names
T3D, IMD19, CD3DELTA, CD3-DELTA

Full Name
CD3 Delta Subunit of T-Cell Receptor Complex

Introduction
CD3D is a crucial subunit of the T-Cell Receptor (TCR) complex, which is responsible for initiating T cell activation. Targeting a component of the TCR complex could theoretically be used to target malignant T cells that express this protein, or as a strategy for non-traditional T cell engagement.

Targeting Diseases
T-Cell Acute Lymphoblastic Leukemia (T-ALL), T-Cell Lymphoma

UniProt ID
P04234

Gene ID
915

Target Species
Human

Antibody Clone
F2B

scFv-Host Species
Human

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

VivoCAR™ Anti-CD3D (F2B) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX66)

Target Information

Customer Reviews and Q&As

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