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Anti-CAIX (AB-MN-21.6.1)-CD86+FcγRI CAR-MA (CARMA-W0188)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-CAIX chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human CAIX and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CAIX antibody (AB-MN-21.6.1) linked to CD86+FcγRI signaling domains. The vector product was designed for the treatment of Metastatic clear cell renal cell carcinoma (ccRCC).

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Details

  • Target
  • CAIX
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Metastatic clear cell renal cell carcinoma (ccRCC)
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • AB-MN-21.6.1
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • CD86+FcγRI
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(CD86+FcγRI)
  • Discription of Signaling Cassetes
  • The cytoplasmic portion of the human CD86 (amino acids 269-329) plus the cytoplasmic part of the human FcγRI (amino acids 314-374) are used as the CAR intracellular signaling domains.
    CD86:
    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.

    FcγRI:
    This protein is a high-affinity Fc-gamma receptor, and plays an important role in the immune response. As a member of the immunoglobulin superfamily (it has two Ig-like domains), FcγRI is expressed on macrophage and other cells, controlling the production of important immune mediators called cytokines that promote inflammation. Fc gamma receptors belong to the group of non-catalytic tyrosine-phosphorylated receptors which share a similar signalling pathway involving phosphorylation of tyrosine residues. The receptors generate signals within their cells through an important activation motif known as an immunoreceptor tyrosine-based activation motif (ITAM). An ITAM is present in the intracellular tail of FcγRIIA, and its phosphorylation induces phagocytosis in macrophages. FcγRI and FcγRIIIA do not have an ITAM but can transmit an activating signal to their phagocytes by interacting with another protein that does. This adaptor protein is called the Fcγ subunit and, like FcγRIIA, contains the two YXXL sequences that are characteristic of an ITAM.

Target

  • Target
  • CAIX
  • Target Species
  • Human
  • Clone
  • AB-MN-21.6.1
  • Host
  • Mouse
  • Gene Name
  • Carbonic anhydrase 9
  • Introduction
  • Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
  • Alternative Names
  • MN; CAIX; CA9; Carbonic anhydrase 9

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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