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Anti-CEA (F11-39)-CD147 CAR-MA (CARMA-W0235)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-CEA chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human CEA and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CEA antibody (F11-39) linked to CD147 signaling domains. The vector product was designed for the treatment of Ovarian, gastrointestinal, colorectal, hepatocellular carcinoma (HCC).

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Details

  • Target
  • CEA
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Ovarian, gastrointestinal, colorectal, hepatocellular carcinoma (HCC)
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • F11-39
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • CEA
  • Target Species
  • Human
  • Clone
  • F11-39
  • Host
  • Mouse
  • Gene Name
  • CEA cell adhesion molecule 5
  • Introduction
  • This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
  • Alternative Names
  • CEA; CD66e; CEA cell adhesion molecule 5; CEACAM5

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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