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Anti-CEA KIR CAR (XW-264) Vector (XS-1122-YF264)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-CEA KIR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human CEA. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv (XW-264) of anti-CEA antibody linked to KIR2DS2 TM & ICD and DAP12. The vector product was designed for the discovery and development of cellular therapy against Colon Cancer. This KIR signaling is designed to prolong KIR-CAR T cells' efficacy period by coupling the receptor chains during activation and decoupling them during rest, avoiding T cell exhaustion even in challenging solid-tumor environments.

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Details

  • Target
  • CEA
  • Targeting Cell Type
  • T Cell
  • Targeting Diseases
  • Colon Cancer
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-KIR2DS2 TM&ICD-2A-DAP12
  • Discription of Signaling Cassetes
  • The dual binding and signal chains are designed to come together to form a single receptor complex when bound to a tumor target. Dual chain signaling provides prolonged T cell activation even in challenging solid-tumor environments. KIR CAR combines the power of NK cell KIR receptors with the long-term durability of T cells, resulting in a long-lasting and powerful anti-cancer efficacy. KIR CAR-T cells maintained activation and potent cytotoxic activities in solid tumors compared with the CD28 and 4-1BB co-stimulated CD3-ζ -based traditional CAR T cells. The DAP12 signal functions as both activation and co-stimulation for T cells, circumventing the CD3-ζ pathway to allow prolonged KIR-CAR function.

Target

  • Clone
  • XW-264
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • CEA cell adhesion molecule 5
  • Synonyms
  • CD66e, CEA
  • Introduction
  • This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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