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Anti-EGFRvIII (139) h(scFv-CD79β) CBCR, NALM6 (XS-0323-ZP2506)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The integration of synthetic antigen receptors into immune cells has emerged as a powerful therapeutic strategy for treating various diseases, with T cells engineered to express chimeric antigen receptors (CAR) being a notable example for targeted cancer therapy. However, in addition to T lymphocytes, B lymphocytes have also shown promise as immune cells that can be genetically engineered for therapeutic purposes. One such approach involves using CAR-B cells to deliver monoclonal antibodies to tumor sites by targeting specific tumor-associated antigens (TAA).

Creative Biolabs has established a novel chimeric B cell receptors (CBCR) cell platform based on B leukemic cell line NALM6 to investigate the potential of CBCRs as a vehicle for adoptive immune cell therapies. B leukemic cell line NALM6 are genomically modified with the Anti-EGFRvIII CBCR vector (Cat.# XS-0323-ZP794). The expression of CBCR is then detected to confirm the precise integration of the anti-EGFRvIII CBCR into the appropriate locus of the B leukemic cell line NALM6.

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Specifications

  • Species
  • Human
  • Host Cell Type
  • NALM6
  • Shipping
  • Dry ice
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
  • Warnings
  • Avoid multiple freeze/thaw cycles
  • Research Use Only
  • For research use only, not for diagnostic or therapeutic use.
  • Quality Control
  • Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).
  • Sterility Testing
  • All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

CAR Design

  • Target
  • EGFRvIII
  • Target Species
  • Human
  • scFv Clone
  • 139
  • scFv Host Spcies
  • Mouse
  • CAR Construction
  • scFv-CD79β
  • CAR Signaling Cassetes
  • The peptide CD79 is required for BCR transport to the cell surface and signal transduction. CBCR-engineered B cells equipped with the CD79β signaling domain, upon exposure to antigen, selectively expand and contribute to memory and plasma cell repertoires, not only for the long-term supply of defective proteins from long-lived plasma cells, but also enabling the reactivation of engineered cells from the memory B cell pool by antigen re-exposure.
  • CAR Vector Name
  • pCDCAR1
  • CAR Vector Type
  • Lentivirus

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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