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Anti-UL83 (CPSQEPMSIYVY) TCR, Jurkat Cell Line (TCRJ-YC1312)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-UL83 (CPSQEPMSIYVY) TCR Jurkat cell line is a stable cell line made from the anti-UL83 TCR lentivirus. The recombinant Jurkat T cell was designed to predict the MOA of UL83-TCR, measure the UL83-TCR specificity and screen target cells expressing UL83. And the product can be used to treat cytomegalovirus infection.

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Specifications

  • Cell Background
  • The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.
  • Cell Type
  • T lymphocyte
  • Formulation
  • Containing ≥ 1 X 10*6 / vial frozen cells
  • Cell Purity
  • >95%
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
  • Applications
  • • Screen for activators or inhibitors of CMV signaling in a cellular context
    • Characterize the biological activity of CMV and its interactions with ligands
    • Predict the MOA of the TCR design
    • Screen and validate CMV-expressing target cells
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Warnings
  • Avoid multiple freeze/thaw cycles
  • Research Use Only
  • Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.
  • Quality Control
  • Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
  • Tumorgenicity
  • Positive (In vitro/vivo transformation assay)
  • Oncogenicity
  • Positive (In vitro soft agarose assay and life-time studies )
  • Sterility Testing
  • Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
  • Identity Testing
  • Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
  • Virological Safety Testing
  • A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
  • Genetic Stability Testing
  • We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

TCR Design

  • Target
  • UL83
  • Introduction
  • pp65 is the most abundant tegument protein and the major constituent of extracellular virus particles. However, pp65 is not essential for the production of new infectious virus particles as evidenced in strains that lack the pp65 gene which can still replicate in culture. pp65 is the major tegument protein responsible for modulating/evading the host cell immune response during HCMV infections. pp65 is implicated in counteracting both innate and adaptive immune responses during HCMV infections. pp65's role in immune evasion is largely attributable to its targeting of both humoral and cellular immunity as well as serving as the dominant target antigen of cytotoxic T lymphocytes. It has been demonstrated that pp65 not only prevents immediate-early proteins from being recognized by components of the immune system, but it also inhibits the synthesis of the various components involved in the host cell's immune response. One of the ways in which pp65 counteracts adaptive immunity is through its enzymatic kinase activity. It was shown that pp65 mediates the phosphorylation of viral immediate-early proteins, which blocks their presentation to the major histocompatibility complex class I molecules. The kinase activity of pp65 has also been implicated in causing the degradation of the alpha chain in the major histocompatibility class II cell surface receptor, HLA-DR, via an accumulation of HLA class II molecules in the lysosome. Furthermore, several studies have presented evidence that pp65 is involved in mediating a decrease in the expression of major histocompatibility complex class II molecules. This is significant in that major histocompatibility complex class I and II molecules are responsible for lymphocyte recognition and antigen presentation with class I molecules presenting to cytotoxic T lymphocytes CD8+ and class II molecules to helper T lymphocytes CD4+.
  • HLA
  • HLA-B*35:08
  • Common Name
  • UL83
  • Target Species
  • HCMV
  • TCR Clone
  • r6β
  • TCR-Host Animal
  • Human
  • Vector Name
  • pCDTCR1
  • Vector length
  • ~ 8 kb
  • Vector Type
  • Lentiviral vector
  • Epitope
  • CPSQEPMSIYVY
  • Format
  • Non-Modified TCR

Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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