αβ TCR-γδ T Cell-Specific Response Analysis Services

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Based on the extensive experience in CAR-T and TCR-T therapy services, Creative Biolabs extends its platforms in cell therapy services and provides one-stop αβ TCR-engineered γδ T development services for worldwide customers. We provide comprehensive assays to analyze αβ TCR-γδ T cell-specific responses.

T cell receptor (TCR) gene transfer is an effective strategy to produce therapeutic T cells with clinical benefit in the treatment of cancer. As the γ and δ TCR chains do not mispair with transferred α or β chains, transferring γδ TCR into αβ T cells or αβ TCR into γδ T cells is a strategy to avoid the risk of TCR mispairing. TCR-γδ T cells gain robust anti-tumor activities and release cytokines in both αβ- and γδ TCR-independent manners. These αβ TCR and CD8-transduced γδ T cells reacted more rapidly to the target cells compared to the conventional αβ T cells.

γδ T cells transduced with both αβ TCR and CD8 can recognize antigen-specific peptide/MHC and specific kill target cells. We can assess the capacity of αβ TCR and CD8 co-transduced γδ T cells to recognize targets through the transduced αβ TCR.
αβTCR-γδ T cells are stimulated either through αβ TCR or γδ TCR to rapidly proliferate, and produce cytokines and granzyme. We can provide a full range of validation assay services to assess killing activity against the target cells.

Fig.1 αβ TCR-γδ T cells activation and effector function.

We offer the following in vivo assays. For other assays, please contact us to learn about our customization services.

TCR Specificity Assay

It is well-known that TCRs specifically recognize the peptide/MHC. We provide high-quality in vitro assays to test the specificity of engineered T cells for interested antigens.

Cytokine Release Test

Successfully modified T cells can secrete higher levels of cytokines (such as IFNγ, IL-2, and TNF). Our cytokine release assay can be customized to meet the demands of your program. For your different needs, we can provide you with multi-factor detection services and the different detection methods: flow cytometry, enzyme-linked immunospot assay (ELISPOT), enzyme-linked immunosorbent assay (ELISA), or others.

Cytotoxic Assay

The αβ TCR engineered γδ T cell is a bi-potential T cell that can show cytotoxicity against tumor cells in response to αβ cell and γδ T cell ligands. We provide comprehensive cytotoxicity test services, including LDH cytotoxicity test, flow cytometry-based cytotoxicity test, chromium-51 release assay, and impedance-based label-free real-time cytotoxicity assays.

Cell Proliferation Test

γδ T cells that are engineered to express an MHC class I-restricted TCR are capable of antigen-specific survival or proliferation upon interaction with antigen-expressing APCs. We can evaluate this activation and proliferation process through our different proliferation assay approaches.

If you are interested in these above services or others, please feel free to contact us to learn more.

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