CIK Apoptosis Analysis Service

Cytokine-induced killer (CIK) cells are a heterogeneous population of highly cytotoxic immune effector cells. Creative Biolabs has developed a series of assays to precisely evaluate apoptotic processes in CIK cells, utilizing cutting-edge technology and a deep understanding of cellular biology.

Fig.1 CIK immunotherapy.¹

Importance of Apoptosis Analysis in CIK Cells

CIK cell therapy represents a promising approach in cancer treatment, leveraging the potent cytotoxic capabilities of CIK cells against tumor cells. The apoptotic pathways within CIK cells are crucial for regulating their life cycle and function, making apoptosis analysis an essential component of CIK cell studies. The efficacy of CIK therapy can be influenced by the phenomenon of CIK cell apoptosis, particularly through mechanisms involving activation-induced cell death (AICD). A study indicates redirection of CIK cells to tumor targets by CD3 leads to an increase in both CIK cell proliferation and apoptosis.

Comprehensive CIK Apoptosis Analysis Service

CIK cells are a heterogeneous population of highly cytotoxic immune effector cells, making them an ideal model for apoptosis studies. Creative Biolabs has developed a series of assays to precisely evaluate apoptotic processes in CIK cells, utilizing cutting-edge technology and a deep understanding of cellular biology. We utilize advanced techniques such as flow cytometry to analyze markers of apoptosis, including Annexin V binding, caspase activation, and DNA fragmentation. These markers provide a comprehensive view of apoptotic events in CIK cells. Our service is suited to distinct phases of apoptosis.

• Flow Cytometry

We use fluorescence-labeled annexin V and propidium iodide (PI) staining to distinguish early apoptosis, late apoptosis, and necrosis. This quantitative method offers high sensitivity and specificity.

• TUNEL Assay

The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay is employed to detect DNA fragmentation, a hallmark of apoptosis.

• Caspase Activity Assays

Measurement of caspase enzymes, such as caspase-3 and caspase-7, provides insights into the intrinsic and extrinsic apoptotic pathways in CIK cells.

Key Advantages of Our CIK Apoptosis Analysis Service

• Customizable Solutions: We offer customizable testing protocols tailored to meet specific research needs
• State-of-the-Art Technology: Our CIK Apoptosis Analysis Service employs cutting-edge technologies that allow for multi-parameter analysis.
• Expert Guidance: Our team of experienced scientists provides comprehensive support, from experimental design to data interpretation.
• Rapid Turnaround: We prioritize efficiency to meet project timelines without compromising quality.

At Creative Biolabs, our team of experts combines years of experience with state-of-the-art technologies to provide comprehensive apoptosis analysis solutions. By partnering with us, researchers can gain actionable insights into the apoptotic mechanisms affecting CIK cells, empowering the development of innovative cancer therapies. Contact us today to discover how our CIK Apoptosis Analysis Service can support and enhance your research endeavors.

Reference

1. Gao, Xingchun, et al. "Cytokine-induced killer cells as pharmacological tools for cancer immunotherapy." Frontiers in immunology 8 (2017): 774. Distributed under Open Access license CC BY 4.0, without modification.