CIK Cytokine/Chemokine Analysis Service

Cytokine-induced killer (CIK) cells are a unique population of immune cells known for their potent anti-tumor properties and ability to target a diverse range of cancer cells. At Creative Biolabs, we have tailored our cytokine/chemokine analysis service to provide researchers with detailed insights into the secretory profile of CIK cells, which is pivotal for the development of effective immunotherapeutic strategies.

Cytokine/Chemokine Secretion by CIK cells

CIK cell therapy represents a promising immunotherapeutic approach leveraging the potent cytotoxic activity of CIK cells against cancer. These cells are a heterogeneous population of T lymphocytes, predominantly CD3+CD56+ subsets, generated by ex vivo stimulation with cytokines such as IFN-γ, IL-2, and anti-CD3 antibodies. CIK cells exhibit robust antitumor effects through a non-MHC-restricted mechanism, making them effective against various malignancies. In addition to their cytotoxicity, CIK cells secrete a diverse range of cytokines and chemokines, including IFN-γ, TNF-α, and IL-2, which enhance immune responses by recruiting and activating other immune cells in the tumor microenvironment. Understanding the cytokine and chemokine secretion profiles of CIK cells is crucial for optimizing their therapeutic potential, tailoring treatments to individual patients, and mitigating potential adverse effects.

Fig.1 Anti-cancer activity of CIK cells.¹

Comprehensive CIK Cytokine/Chemokine Profiling Service

Creative Biolabs has developed a comprehensive service platform for the analysis of cytokine/chemokine profiles in CIK cells. The service covers a wide array of cytokines and chemokines, including but not limited to interleukins (IL-6, IL-10), TNF-α, IFN-γ, and CCLs. Our platform aims to provide reliable and precise profiling which is crucial for research and therapeutic applications involving immune responses and inflammation regulation.

Our advanced platforms employ various techniques for cytokine and chemokine analysis, ensuring precision and reliability. These methods include:

• ELISA: For targeted analysis, we provide high-sensitivity ELISA to quantify specific cytokines/chemokines.
• Flow Cytometry: Combined with bead-based technologies, this method provides comprehensive profiling and quantification, allowing researchers to assess the secretome of CIK cells.
• Multiplex Assays: These allow simultaneous measurement of multiple cytokines and chemokines in a single run, enhancing throughput and efficiency.

Applications of CIK Cytokine/Chemokine Analysis

• Assessing the efficacy of CIK cells
• Optimizing CIK cell-based therapies for cancer and other diseases
• Exploring the immune modulatory effects of CIK cells
• Identifying key molecules associated with CIK cell efficacy and safety

Key Advantages of Our Service

• Cutting-Edge Technologies: Creative Biolabs utilizes advanced platforms, including ELISA, multiplex bead-based assays, and flow cytometry, for the accurate quantification and profiling of cytokines and chemokines.
• Broad Coverage: Detection of multiple cytokines and chemokines in a single sample, saving time and resources.
• High Sensitivity: Ability to detect low-abundance molecules with precision.
• Rapid Turnaround Time: Our streamlined processes ensure fast delivery without compromising quality.

Reference

1. Li, Celine Man Ying, et al. "Clinical application of cytokine-induced killer (CIK) cell therapy in colorectal cancer: Current strategies and future challenges."Cancer Treatment Reviews (2023): 102665. Distributed under Open Access license CC BY 4.0, without modification.