CIK Immune Cell Subtype Analysis Service

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Welcome to our CIK (Cytokine-Induced Killer) therapy platform, where we are committed to advancing the forefront of cancer immunotherapy research. Creative Biolabs offers specialized preclinical CRO analysis services for CIK immune cell subtypes, designed to provide robust support for your research and clinical development initiatives.

Services

Analyzing the subtypes of CIK immune cells plays a key role in cancer immunotherapy. It can uncover the functions and characteristics of different cell types to enhance therapeutic outcomes. Nowadays, the analysis of CIK immune cell subtypes is significantly impacted by Creative Biolabs in various ways:

Firstly, functional assessments of cells can evaluate their anti-tumor capabilities by analyzing the ratios and functions of different subtypes, such as NK cells and T cells.
Secondly, personalized therapy is made possible by aligning treatment plans with the unique immune cell profiles.
Furthermore, our subtype analysis can also help assess immune responses and survival durations, providing valuable insights for new CIK therapy discovery.
In addition, monitoring changes in immune status is essential for the early detection of issues related to immune suppression or resistance, which can inform adjustments in CIK therapy strategies.
Lastly, investigating the variations in CIK cell subtypes contributes to a deeper understanding of new immune mechanisms and potential therapeutic targets, thereby advancing scientific research on cancer immunotherapy.

Overview of Multiple Approaches Aiming at ImprovCytokine-Induceduced Killer (CIK) Cell Therapy in Lymphoma. Fig.1 Cytokine-induced Killer (CIK) Cell Therapy.^1,3

Process

Currently, Creative Biolabs is developing and refining analytical techniques for CIK immune cell subtypes to provide comprehensive data support for the development and evaluation of CIK therapy. Here are some key technical approaches:

Cell Isolation and Culture

In our labs, target cells are extracted through the separation of peripheral blood mononuclear cells (PBMC) using density gradient centrifugation. These cells are then expanded using specific culture media and cytokines (such as IL-2, IFN-γ) to produce a large quantity of CIK cells.

Phenotypic Analysis of Cell Subtypes

Flow cytometry is employed to analyze cell surface markers, allowing the identification of various CIK cell subtypes (like NK-like CIK and T-like CIK). Meanwhile, using multiplex staining techniques enables the simultaneous detection of multiple surface markers to gain deeper insights into the functional characteristics of the cells.

Functional Assays

Cytotoxicity assays (such as LDH release assay or CCK-8 assay) are performed by our scientists to assess the tumor-killing capabilities of different CIK cell subtypes. Additionally, cytokine release assays (like ELISA) are conducted to evaluate the activation status of immune cells.

Gene Expression Analysis

Real-time quantitative PCR (qPCR) and RNA-seq technologies are used to analyze the gene expression profiles of various CIK cell subtypes, identifying key genes and pathways associated with immune activity.

Combination Therapy Evaluation

We carry out the combined application of CIK cells with other immunotherapies (such as CAR-T cells, and immune checkpoint inhibitors) to evaluate the synergistic effects and therapy potential of different CIK cell subtypes.

Animal Model Studies

At Creative Biolabs, in vivo experiments are conducted using many animal models (such as mouse and rat) to observe the anticancer effects of various CIK cell subtypes and their interactions with host immune responses, providing reliable data for forthcoming human trials.

Flow Cytometric Quality Assessment of CIK Cells During Ex Vivo Expansion. Fig.2 Flow Cytometric Analysis of CD4+ T Cells in Various CIK Cell Populations.^2,3

Advantages

Diverse CIK Subtype Analysis	Creative Biolabs offers comprehensive and systematic analysis tailored to various subtypes of CIK cells, providing a complete solution customized for your project.
Fully Customized Services	We deliver an all-in-one service from sample collection and processing to data analysis, ensuring your project advances efficiently and on schedule while addressing your specific requirements.
Expert Team Support	Our team of scientists is available to provide you with specialized guidance and technical assistance, ensuring the quality and reliability of your research.
High-Sensitivity Detection	Equipped with multiple leading international technology platforms, we guarantee accurate and sensitive detection of CIK cell subtypes, significantly enhancing the credibility of the analysis results.
Rapid Response Mechanism	Understanding the urgency of CIK projects, Creative Biolabs has established an efficient project management process that allows for quick adaptation to client needs and changes, accelerating the research timeline.
Integration of Top Class Technologies	Our staff continually adopts new technologies and methodologies, ensuring your research remains at the forefront of the industry, and helping you stand out in a competitive landscape.

Creative Biolabs can assist clients in understanding the biological characteristics and therapeutic potential of CIK immune cell subtypes, thus laying a solid foundation for CIK therapy development. We offer clients high-throughput services for cell function assessment, cell phenotype analysis, and cytokine secretion evaluation, ensuring efficiency and accuracy in experimental results. We look forward to collaborating with you to advance the innovation and development of CIK immunotherapy. If you are interested in our services or have any related inquiries, please feel free to contact us.

References

Zhang, Ying, et al. "Clinical studies on cytokine-induced killer cells: lessons from lymphoma trials." Cancers 13.23 (2021): 6007.
Liu, Shaochuan, et al. "CD4+ T cells are required to improve the efficacy of CIK therapy in non-small cell lung cancer." Cell death & disease 13.5 (2022): 441.
Distributed under Open Access license CC BY 4.0, without modification.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.