CIK In Vivo Antitumor Activity Test Service

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Creative Biolabs offers specialized in vivo antitumor activity analysis services for cytokine-induced killer (CIK) cells in preclinical studies. We understand that accurate and reliable experimental data are significant for the successful development of new CIK therapies. To meet the diverse needs of our clients, we have developed a range of unique technical platforms and analytical methods.

Services

CIK therapy, an emerging form of immunotherapy, fights tumors by enhancing the body's immune response. Within this treatment, the assessment of in vivo antitumor activity plays an important role. This analysis quantitatively evaluates the ability of CIK cells to eliminate tumor cells. Moreover, the evaluation of in vivo antitumor activity offers essential data to support personalized treatment approaches.

Creative Biolabs provides a series of analytical services for evaluating the in vivo antitumor activity of CIK cells during the preclinical phase, which significantly supports tumor immunotherapy discovery. We are capable of rapidly and accurately assessing and enhancing the ability of CIK cells to inhibit tumor growth and improve therapy prognosis. Our approach involves designing experiments tailored to specific tumor models to observe the migration, proliferation, and antitumor effects of CIK cells in vivo. Additionally, we employ advanced cell culture techniques and cytokine stimulation to ensure that CIK cells attain sufficient activity in vivo before injecting them into tumor models for observation. Finally, Creative Biolabs conducts regular monitoring of tumor growth, including changes in tumor volume and survival rate analysis. Utilizing imaging techniques, histological examinations, and flow cytometry, our team can comprehensively evaluate the antitumor effects of CIK cells and elucidate their mechanisms of action.

Cancer Recognition and Anti-cancer Activity of CIK Cells. Fig.1 CIK Cells for Cancer Immunotherapy.^1,3

Procedures

At Creative Biolabs, our CIK in vivo antitumor activity testing assay has been used as a popular analysis approach grounded in cellular immunotherapy, designed to evaluate the efficacy of CIK therapy against various malignancies. Our analysis primarily focuses on examining the antitumor effects of CIK cells through in vivo assays.

In conducting CIK in vivo antitumor activity tests, we begin by collecting peripheral blood mononuclear cells from various samples. These cells are then stimulated with specific cytokines, such as IL-2 and IFN-γ, under controlled culture conditions to promote the generation of highly active CIK cells. Following the cultivation period, these CIK cells are injected into established tumor models, such as mice or rats, to assess their antitumor efficacy within a living organism. Our CIK in vivo antitumor activity analysis service evaluates multiple parameters, including changes in tumor volume, tumor growth inhibition rates, survival times of the animals, and the infiltration of immune cells. By comparing the results between the experimental and control groups, we can accurately assess the antitumor activity of the CIK cells.

Tumor Necrosis and Infiltration by CIK In Vivo. Fig.2 IHC Staining Analysis of CIK Tumor Infiltration In Vivo.^2,3

Advantages

Comprehensive Animal Model Repository: Creative Biolabs boasts a diverse array of tumor animal models, including patient-derived mouse models, which simulate the human tumor microenvironment, providing a solid foundation for assessing the anti-tumor efficacy of CIK cells.
Customized Solution Plans: We offer tailored experimental designs and protocols to meet specific client requirements. Whether it's the source of CIK cells, their developmental pathways, or the administration methods, we create bespoke solutions to ensure the most valuable data is obtained.
Multifaceted Evaluation System: Our scientists focus not only on changes in tumor volume but also integrate pathological assessments of tumor tissues, immunohistochemical analyses, and molecular biology evaluations, allowing for a comprehensive understanding of the anti-tumor mechanisms of CIK cells.
High-Throughput Data Analysis: Based on various advanced high-throughput data analysis systems, our labs can rapidly and accurately process experimental data, producing easily interpretable reports that empower clients to make informed scientific decisions in the shortest possible time.
Expert Team Support: Our team is composed of professionals with extensive experience in in vivo CIK cell analysis, ready to provide expert consultation and technical support at any stage, ensuring the smooth progression of your project.

Creative Biolabs provides high-quality in vivo analysis services for the antitumor activity of CIK cells in preclinical studies, helping you to take a strong and confident step forward in your cancer immunotherapy journey. We can accurately detect the antitumor effects of CIK cells across many preclinical models, providing you with dependable data support. If you are interested in our CIK antitumor activity analysis services, please feel free to contact us for more information or to request a customized solution. We look forward to offering comprehensive support and services for your research needs!

References

Li, Celine Man Ying, et al. "Clinical application of cytokine-induced killer (CIK) cell therapy in colorectal cancer: Current strategies and future challenges." Cancer Treatment Reviews (2023): 102665.
Capellero, S., et al. "Preclinical immunotherapy with Cytokine-Induced Killer lymphocytes against epithelial ovarian cancer." Scientific Reports 10.1 (2020): 6478.
Distributed under Open Access license CC BY 4.0, without modification.

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