Human Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-105 Plex (CAR-K-LX-005)

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Product Name
Human Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-105 Plex

Cat. No.
CAR-K-LX-005

Product Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Components
Rectangular 4-Well Multi-dish.
4 Human Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines.
Array Buffer 4.
Array Buffer 6.
Chemi Reagent 1.
Chemi Reagent 2.
Detection Antibody Cocktail, Human XL Cytokine Array.
Streptavidin-HRP.
Transparency Overlay Template.
Wash Buffer Concentrate (25X).

Assay Type
Multiplex

Species Reactivity
Human

Sample Type (General)
Cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Shipping Condition
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage Conditions
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Dtection Coverage
Adiponectin/Acrp30, IFN-gamma, CCL2/MCP-1, Angiogenin, IGFBP-2, CCL7/MCP-3, Angiopoietin-1, IGFBP-3, M-CSF, Angiopoietin-2, IL-1 alpha/IL-1F1, MIF, Apolipoprotein A1, IL-1 beta/IL-1F2, CXCL9/MIG, BAFF/BLyS/TNFSF13B, IL-1ra/IL-1F3, CCL3/CCL4, MIP-1 alpha/beta, BDNF, IL-2, CCL20/MIP-3 alpha, CD14, IL-3, CCL19/MIP-3 beta, CD30, IL-4, MMP-9, CD31/PECAM-1, IL-5, Myeloperoxidase, CD40 Ligand/TNFSF5, IL-6, Osteopontin (OPN), Chitinase 3-like, IL-8, PDGF-AA, Complement Component C5/C5a, IL-10, PDGF-AB/BB, Complement Factor D, IL-11, Pentraxin 3/TSF-14, C-Reactive Protein/CRP, IL-12 p70, CXCL4/PF4, Cripto-1, IL-13, RAGE, Cystatin C, IL-15, CCL5/RANTES, Dkk-1, IL-16, RBP4, DPPIV/CD26, IL-17A, Relaxin-2, EGF, IL-18 BPa, Resistin, CXCL5/ENA-78, IL-19, CXCL12/SDF-1 alpha, Endoglin/CD105, IL-22, Serpin E1/PAI-1, EMMPRIN, IL-23, SHBG, Fas Ligand, IL-24, ST2/IL1 R4, FGF basic, IL-27, CCL17/TARC, KGF/FGF-7, IL-31, TFF3, FGF-19, IL-32 alpha/beta/gamma, TfR, Flt-3 Ligand, IL-33, TGF-alpha, G-CSF, IL-34, Thrombospondin-1, GDF-15, CXCL10/IP-10, TIM-1, GM-CSF, CXCL11/I-TAC, TNF-alpha, CXCL1/GRO alpha, Kallikrein 3/PSA, uPAR, Growth Hormone (GH), Leptin, VCAM-1, HGF, LIF, VEGF, ICAM-1/CD54, Lipocalin-2/NGAL, Vitamin D BP

Detection method
Fluorescent

Product Features
Avaliability: able to detect 105 human cytokines simultaneously;
No specialized equipment requirement;
Compatible with LI-COR and chemiluminescence detection.

Principle of the Assay
The Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Human Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-105 Plex (CAR-K-LX-005)

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