CellRapeutics™ Transfection Kit(CART-020CL)
CellRapeutics™ Transfection Kit
The Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. CellRapeuticsTM DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac, pBAC, or pTriEx vectors, is cotransfected with CellRapeuticsTM DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The Transfection Kit contains the CellRapeuticsTM DNA, Insect GeneJuice Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and Vector Medium, for optimal transfection performance.
• 0.5 µg CellRapeuticsTM DNA
• 50 µl Insect GeneJuice Transfection Reagent
• 2 x 1 ml Sf9 Cells
• 1 L Vector Insect Cell Medium
• 2 µg Transfection Control Plasmid
Multiple Toxicity Values, refer to MSDS
Multiple Storage Conditions OK to freeze
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.