CAR-Manu™ Annexin V Apoptosis Detection Kit, PE, with 7-AAD (CAR-K-Z026)

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Product Name
CAR-Manu™ Annexin V Apoptosis Detection Kit, PE, with 7-AAD

Cat. No.
CAR-K-Z026

General Description
The kit has been specifically designed for the identification of apoptotic cells.

Product Summary
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins, which binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is usually only found in intracellular leaflets of the plasma membrane of healthy cells, but during early apoptosis, the asymmetry of the membrane disappears and PS translocates to the outer leaflet. Annexin V labeled with a fluorescent dye can then be used to specifically target and identify apoptotic cells. It is recommended to use Annexin V binding buffer for Annexin V staining. Annexin V binding alone cannot distinguish between apoptotic cells and necrotic cells. To help distinguish between necrotic and apoptotic cells, we recommend using our 7-amino-actinomycin D (7-AAD) solution. Early apoptotic cells will exclude 7-AAD, while late apoptotic cells will be stained because these dyes enter the nucleus bound to DNA.

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is effectively excluded by intact cells. During flow cytometry analysis, it can be used for DNA analysis and dead cell identification. When excited by a 488 laser, 7-AAD fluorescence was detected in the far red range of the spectrum (650 nm long-pass filter).

Components
0.5 ml of PE Annexin V
0.5 ml of 7-AAD Viability Staining Solution
50 ml of Annexin V Binding Buffer

Species Reactivity
All mammalian species

Storage Conditions
Store between 2°C and 9°C. Do not freeze.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

CAR-Manu™ Annexin V Apoptosis Detection Kit, PE, with 7-AAD (CAR-K-Z026)

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