MHC Class II Protein expressed Artificial T Cell Stimulator Development Service

The Crucial of MHC Class II Protein expressed Artificial T Cell Stimulator Development

Artificial T cell stimulators are engineered platforms used to activate and expand T cells by attaching T cell-stimulating proteins onto specific carriers. Among these proteins, MHC class II molecules play a crucial role as they typically present antigens and are normally present on professional antigen-presenting cells. When these molecules are engineered and paired with suitable peptides, and integrated with artificial T cell stimulators, they offer a model to explore T cell signaling intricacies and represent a potential strategy for developing innovative immunotherapies.

Fig.1 Antigen presentation routes using MHC-II.^1,3

MHC Class II Protein expressed Artificial T Cell Stimulator Development Service at Creative Biolabs

Creative Biolabs offers a cutting-edge service in developing MHC class II protein-expressed artificial T cell stimulators aimed at advancing and optimizing T cell therapies for clients worldwide. We utilize efficient vectors or alternative delivery methods to integrate recombinant MHC class II molecules, laden with diverse target antigen peptides, into cell lines or biocompatible substrates. This enables the creation of tailored artificial T cell stimulators with enhanced T cell activation capabilities, eliminating the need for external cytokines or feeder cells. From initial design to final validation, our comprehensive, one-stop service ensures clients receive high-quality artificial T cell stimulators that meet their expectations, all within a rapid turnaround time.

In addition, we employ state-of-the-art techniques such as advanced flow cytometry, ELISA, and qPCR to conduct comprehensive, multi-dimensional testing on artificial T cell stimulators. This rigorous testing ensures their effectiveness in antigen presentation and T cell activation. Furthermore, we provide detailed, customized quality control reports that meticulously document the production process, testing results, and associated data of the artificial T cell stimulators, offering comprehensive quality assurance to our clients. At Creative Biolabs, we are committed to delivering exceptional service, and dedicated to helping you achieve your scientific objectives with excellence.

Benefits for You

• Robust artificial T cell stimulator development platform that adheres to the highest industry requirements
• Customized solutions to meet various needs.
• Comprehensive service process from design to delivery.
• Rigorous quality control systems.
• One-stop service process.

Representative Data

Summary: This study explored a novel nanoparticle platform for efficiently expanding antigen-specific CD4+ T cells in vitro. By mimicking the function of antigen-presenting cells (APCs), these nanoparticles, displaying major histocompatibility class II (MHC II) molecules, effectively stimulated the growth of CD4+ T cells, including rare subsets, when combined with soluble costimulatory signals. This approach demonstrated the potential to overcome limitations in current CD4+ T cell expansion techniques, paving the way for more consistent and scalable CD4+ T cell-based therapies and facilitating deeper investigations into CD4+ T cell biology.

Fig.2 Functional antigen-specific murine CD4+ T lymphocytes are stimulated by MHC II artificial T cell stimulators.^2,3

Fig.3 CD4+ T cell cytotoxicity stimulated by MHC II artificial T cell stimulators.^2,3

Creative Biolabs builds on years of focused study in T cell biology, which provides a solid theoretical foundation and substantial practical experience. This distinct incorporation enables us to design and manufacture high-quality artificial T cell stimulators that are exactly tailored to your specific research or therapeutic objectives. Contacting us for a thorough consultation to go over your needs will allow you to explore the whole range of options. Please contact us for an in-depth consultation to discuss the entire variety of options and your specific requirements.

References

1. Couture, Alexandre, et al. "HLA-class II artificial antigen presenting cells in CD4+ T cell-based immunotherapy." Frontiers in Immunology 10 (2019): 1081.
2. Isser, Ariel, et al. "Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy." Nature Communications 13.1 (2022): 6086.
3. Distributed under Open Access License CC BY 4.0, without modification.