Nicotinamide induced NK Cell Expansion Service

The clinical application of NK cells is often constrained by the limitations of conventional expansion methods, which frequently yield products with insufficient cell numbers, functional exhaustion, and impaired in vivo persistence. To address these challenges, Creative Biolabs' Nicotinamide-Induced NK Cell Expansion Technology provides a novel platform that fundamentally enhances NK cell fitness through metabolic reprogramming. By modulating cellular metabolism, Nicotinamide (NAM) not only promotes robust, large-scale ex vivo expansion but also concurrently upregulates key activating receptors and augments mitochondrial fitness, resulting in NK cells with superior cytotoxicity and enhanced survival in the immunosuppressive tumor microenvironment.

Introduction

NK cells are potent effector lymphocytes vital for cancer immunosurveillance. Their innate tumor-killing capability and low risk of make allogeneic cell therapy highly promising. However, clinical efficacy has historically been limited by poor expansion yields and short persistence due to metabolic exhaustion. NAM, a form of vitamin, solves this by acting as a kinase inhibitor and metabolic conditioner. By stabilizing the transcription factor, enhances the expression of selectin and glucose flux, resulting in a robust, high-purity product with superior persistence and resistance to tumor evasion mechanisms.

Fig.1 Modulation of NK cell function through NAM, GSK-3 inhibition and HSPs.¹

Nicotinamide-Induced NK Cell Expansion Technology at Creative Biolabs

Creative Biolabs' Nicotinamide-Induced NK Cell Expansion Technology provides specific, high-quality deliverables by leveraging metabolic and kinetic controls to produce reliable cells. We deliver a product that is not only highly expanded but is functionally pre-conditioned for superior activity within the immunosuppressive tumor microenvironment.

What we can offer

We offer a complete customizable platform designed to deliver reliable and effective cell products and development services tailored to your specific oncology targets.

Our Service Process

We implement a streamlined, multi-stage process designed for maximum yield, high purity, and optimal functionality, making the resulting cell products ideally suited for downstream engineering or direct clinical application.

Required starting materials:

• Peripheral Blood Mononuclear Cells (PBMCs).
• The target CAR construct sequence (if required for engineering).

Key Steps Involved:

Final Deliverables:

• Cryopreserved or fresh NK cell product.
• Comprehensive Release Report: Detailed documentation including cell count, viability, T cell contamination level and sterility results.
• In Vitro Functional Cytotoxicity Data: Report showing target cell lysis and activation marker expression in co-culture

Key Advantages

• Superior Post-Engineering Viability: The dual kinase-inhibitory effect of our culture supplement significantly mitigates cellular stress, leading to enhanced cell survival and viability following complex genetic manipulations.
• Fully Customizable Genetic Modification: We provide customized CAR design, precision gene knockout and cytokine expression optimization to tailor the therapeutic to your specific target antigen and safety requirements.
• Advanced Immune Evasion Resistance: The culture process intrinsically downregulates inhibitory checkpoints like NKG2A and TIGIT, generating NK cells that are inherently more potent and effective within the immunosuppressive tumor microenvironment.

FAQ

Why Choose Us?

Moving beyond simple expansion, our platform is dedicated to engineering the next generation of therapeutic cells through advanced metabolic and genetic reprogramming. We specialize in the creation of metabolically and genetically optimized cellular products, engineered for enhanced potency, persistence, and clinical efficacy. Our integrated platform is built upon a foundation of evidence-based mechanisms and rigorously validated protocols, ensuring a streamlined and de-risked pathway from preclinical discovery to clinical-scale manufacturing.

Customer Reviews

"The low-cell contamination we consistently observe with Creative Biolabs' Nicotinamide-Induced NK Cell Expansion Technology, contrasted against previous feeder-cell or protocols, is a major advantage for readiness. It eliminates significant downstream purification bottlenecks."— L. B*****tt.

"Using Creative Biolabs' Nicotinamide-Induced NK Cell Expansion Technology in our research has significantly improved the expression profile, which is critical for our xenograft models where superior lymph node homing is required. The consistency is unmatched."— J. S*****an.

How to contact us?

To address the critical translational challenges of cell yield and durability for your specific program, connect with our specialists to define a tailored development path.

Reference

1. Islam, Rasa et al. "Enhancing a Natural Killer: Modification of NK Cells for Cancer Immunotherapy." Cells vol. 10,5 1058. Distributed under Open Access License CC BY 4.0, without modification. https://doi.org/10.3390/cells10051058.