NKT TCR Design and Construction Service

γδ T cells have recently gained considerable attention as an attractive tool for cancer adoptive immunotherapy. NKT TCR gene-modified γδ T cell-based therapy represents a novel therapy against cancer. As a leading cell therapeutics development solution provider, Creative Biolabs has established a platform of engineering γδ T cells with CARs and TCRs.

Similar to γδ T cells, invariant natural killer T cells (iNKT cells, type I) are a rare subset of T cells bridging innate and adaptive immunity. The vast majority of iNKT cells express an identical TCRα chain, Vα14-Jα18 in mice, Vα24-Jα18 in humans. However, these cells have diverse TCR β-chains, including Vβ8, Vβ7, and Vβ2 in mice and Vβ11 in humans, joined to a range of TCR Dβ and Jβ genes. NKT TCR ligands are endogenous or microbial glycolipids that are presented by the nonpolymorphic MHC class I-like molecule CD1d. iNKT cells can rapidly produce both IFN-γ and IL-4 after ligand stimulation.

Fig.1 Modes of NKT cell activation upon bacterial infection.¹

Generally, to construct modified TCR, the sorted single human iNKT cells are subjected to TCR cloning using an established single-cell TCR cloning technology. A validated pair of NKT TCR α and β chain genes are selected and the NKT TCR gene is cloned into the vector for further development. With abundant experience in cell bioengineering during the past decades, we have the ability to provide TCR generation, vector construction and gene transduction system services for the process of TCR modified γδ T cells.

Advantages

• Streamline management to short project cycle
• Extensive experience in TCR generation and vector design
• Customize services for customers according to their special needs
• An excellent expert team support whole-process technical trace and direction

As a reliable partner in γδ T cell engineering, Creative Biolabs aspires to become the trusted solution provider for NKT TCR-γδ T cell therapy development. Based on state-of-art TCR development platforms and knowledge of NKT cell therapy, we are dedicated to offering a comprehensive list of NKT TCR engineered-γδ T cell development services for research purposes. If you are interested in our services, please feel free to contact us.

Reference

1. Vogt, Stefan, and Jochen Mattner. "NKT cells contribute to the control of microbial infections." Frontiers in cellular and infection microbiology 11 (2021): 718350. Distributed under Open Access license CC BY 4.0, without modification.