pCDCAR1 MUC1 h(b2cζ), T(CAR-T-2-M303-CZ)
The vector of anti-MUC1 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human MUC1. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-MUC1 antibody linked to b2c and CD3-zeta signaling domains. And the vector product was designed for the treatment of MUC1-expressing tumors.
Targeting Cell Type
Discription of Signaling Cassetes
The b2c protein belongs to high-sulphur keratin family which comprises at least three proteins, B2A, B2B and B2C. And b2c in chimeric antigen receptors improve T-cell effector functions and enhance anti-tumor activity.
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Mucin 1, Cell Surface Associated
Mucin 1, Cell Surface Associated; Tumor-Associated Epithelial Membrane Antigen; Breast Carcinoma-Associated Antigen DF3; Peanut-Reactive Urinary Mucin; Polymorphic Epithelial Mucin; Carcinoma-Associated Mucin; Mucin 1, Transmembrane; Krebs Von Den Lungen-6; Cancer Antigen 15-3; Episialin; CA 15-3; H23AG; MUC-1; KL-6; PEMT; EMA; PUM; PEM;
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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