pCDCAR1 TAG-72 h(28ζ), T(CAR-T-2-M551-2Z)

Datasheet

The vector of anti-TAG-72 chimeric antigen receptor(CAR) is constructed for the engineering of T cell to target human TAG-72. The T cell is genetically modified through transduction with a lentiviral vector expressing anti-TAG-72 VHH linked to CD28 transmembrane domain/ endodomain and CD3-zeta signaling domains. And the vector product was designed for the treatment of TAG-72 expressing tumor.

Details

Target

TAG-72

Target Species

Human

Targeting Cell Type

T cell

Targeting Diseases

TAG-72 expressing tumor

Generation

Second

Vector Name

pCDCAR1

Vector Length

8kb

Vector Type

Lentiviral

Receptor Construction

VHH-CD28-CD3ζ

Discription of Signaling Cassetes

CD28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatorysignals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells(APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Antibody Clone

CC49

Antibody Host

Humanized

Target

Gene Name

Tumor-associated glycoprotein 72

Official Symbol

TAG-72

Synonyms

TAG-72; mucin antigen; Tumor-associated glycoprotein; Colon cancer; scFv; FcεRIγ; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; CC49

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CAR-T-2-M551-2G	Anti-TAG-72 VHH h(CD28-FcεRIγ) CAR, [T]
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CAR-T-2-M551-CZ	Anti-TAG-72 VHH h(b2c-CD3ζ) CAR, [T]
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CAR-T-2-M325-CZ	Anti-TAG-72 scFv h(b2c-CD3ζ) CART, pCDCAR1
VP-CAR-LC466	Lenti-TAG-72 CAR (scFv-CD4-FcεRIγ, CC49) Viral Particle

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pCDCAR1 TAG-72 h(28ζ), T(CAR-T-2-M551-2Z)

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