VivoCAR™ Anti-CD19 (18B) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX2)

Name: VivoCAR™ Anti-CD19 (18B) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX2
Rating: 5 (1 reviews)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-CD19 (18B) h(CD28-41BB-CD3z) CAR circRNA introduces a specialized alternative for next-generation in vivo engineering, utilizing the 18B clone known for its distinct binding kinetics. The 18B clone is particularly significant for in vivo CAR-T applications where "tonic signaling" and T-cell exhaustion are concerns; its unique epitope recognition can provide a different activation threshold, potentially improving the persistence of circRNA-reprogrammed T cells in dense tumor microenvironments. By delivering this CAR as a circRNA, we enable a transient yet potent pulse of 18B-CAR expression, allowing researchers to explore therapeutic windows that avoid the long-term B-cell aplasia often seen with permanent viral-based CAR-T therapies.

Add to Cart

Target Information

Target
CD19

Common Name
CD19

Alternative Names
B-lymphocyte antigen CD19; B4; CVID3

Full Name
B-lymphocyte antigen CD19

Introduction
A B-cell surface adapter protein that reduces the B-cell receptor signaling threshold. It is the defining and most successful CAR-T cell therapy target for B-cell malignancies like B-ALL and NHL.

Targeting Diseases
Chronic lymphocytic leukemia (CLL)

UniProt ID
P15391

Gene ID
930

Target Species
Human

Antibody Clone
18B

scFv-Host Species
Human

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Related Resources

Overview of CD19-Targeted CAR Cell Therapies

Customer Reviews and Q&As

There are currently no customer reviews or questions for VivoCAR™ Anti-CD19 (18B) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX2). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.