VivoCAR™ Anti-CD22 (m10F4) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX10)

Name: VivoCAR™ Anti-CD22 (m10F4) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX10
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-CD22 (m10F4) h(CD28-41BB-CD3z) CAR circRNA utilizes the high-affinity m10F4 murine clone to achieve precise targeting of the CD22 sialic acid-binding lectin. In the context of in vivo CAR-T, the m10F4 clone is significant for its exceptional sensitivity to low-density antigen expression, ensuring that transiently engineered T cells can detect and eliminate sequestered B-cell malignancies. By integrating this binder with the circRNA platform, the product offers a stable, non-integrating alternative for tackling antigen escape in B-cell leukemia while maintaining a programmable therapeutic window.

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Target Information

Target
CD22

Common Name
CD22

Alternative Names
SIGLEC2, SIGLEC-2, B-lymphocyte cell adhesion molecule, B-cell receptor CD22

Full Name
B-cell receptor CD22

Introduction
CD22 is a Siglec family member (Sialic acid-binding Ig-like lectin) found on the surface of mature B cells and most B-cell malignancies. It functions as an adhesion molecule and a negative regulator of B-cell receptor signaling. CD22 is often targeted in cases of CD19 relapse due to antigen escape.

Targeting Diseases
B-cell Acute Lymphoblastic Leukemia (B-ALL), Non-Hodgkin Lymphoma (NHL)

UniProt ID
P20273

Gene ID
933

Target Species
Human

Antibody Clone
m10F4

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Related Resources

Overview of CD22-Targeted CAR Cell Therapies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.