VivoCAR™ Anti-HMW-MAA (M4-3 ML2) h(CD28-41BB-CD3z) CAR saRNA (XS-0326-XU89)

Name: VivoCAR™ Anti-HMW-MAA (M4-3 ML2) h(CD28-41BB-CD3z) CAR saRNA
SKU: XS-0326-XU89
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-HMW-MAA (M4-3 ML2) h(CD28-41BB-CD3z) CAR saRNA utilizes a self-amplifying RNA backbone to express the M4-3 ML2 clone. In vivo, this clone is essential for targeting high-molecular-weight melanoma-associated antigen on tumor cells and vasculature. The saRNA technology enables a "biological magnification" effect, allowing for robust CAR expression from a low initial load. This ensures that in situ reprogrammed T cells can effectively infiltrate dense melanoma lesions and sustain cytotoxic activity within the immunosuppressive tumor microenvironment.

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CAR Information

Target
HMW-MAA

Target Species
Human

Antibody Clone
M4-3 ML2

scFv Host Species
Human

Receptor Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation CD28-41BB-CD3ζ architecture employs a sequential costimulatory strategy, where the CD28 module drives rapid initial activation and metabolic reprogramming, while the 4-1BB component sustains long-term cellular viability and mitigates exhaustion. This tandem design, anchored by the CD3ζ signaling chain, synergistically amplifies downstream signal transduction, resulting in a balanced T-cell phenotype with robust effector capacity and prolonged durability. The integrated signaling cascade thus optimizes both kinetic expansion and persistence, enhancing antitumor efficacy.

CAR saRNA Properties

Structure
Linear

Cap
Cap 1

PolyA
Segmented PolyA

Product Type
CAR IVT saRNA

Concentration
Lot specific

Buffer
1 mM Sodium Citrate, pH 6.4 (or specialized RNA storage buffer, RNase-free).

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

saRNA Quality Control

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Target Information

Full Name
High Molecular Weight Melanoma-Associated Antigen

Alternative Names
NG2; MCSP; MCSPG; MSK16; CSPG4A; CSPG4; MEL-CSPG

Introduction
HMW-MAA is a large transmembrane proteoglycan overexpressed in over 90% of malignant melanomas and various other solid tumors, where it promotes cell migration, invasion, and tumor angiogenesis.

Targeting Diseases
Melanoma, Neuroblastoma, Certain Sarcomas (Chondrosarcoma)

UniProt ID
Q6UVK1

Gene ID
1464

Case Studies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.