Anti-CD37 (2B8) h(4-1BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
CD37

Targeting Cell Type
T Cell

Targeting Diseases
Lung malignancy

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-4-1BB-CD3ζ

Discription of Signaling Cassetes
4-1BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigennonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

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Published Data

Complete CAR data ELISA

Fig.1 Cytokine IFN-γ release assay.

CAR Construction : 2B8-CD28-CD3ζ

Fig.1 Cytokine IFN-γ release assay.

Interferon-gamma (IFN-γ) secretion by CD37-CAR-T cells against CHO-CD37 cells is significantly higher than against CHO cells.

Golubovskaya, V., Zhou, H., Li, F., Valentine, M., Sun, J., Berahovich, R., ... & Wu, L. (2021). Novel cd37, humanized cd37 and bi-specific humanized cd37-cd19 car-t cells specifically target lymphoma. Cancers, 13(5), 981.

Complete CAR data ELISA

Fig.2 Cytokine IFN-γ release assay.

CAR Construction : 2B8-CD28-CD3ζ

Fig.2 Cytokine IFN-γ release assay.

Secretion of IFN-gamma by CD37-CAR-T cells is significantly higher with Raji cells than with CD37-negative K562 cells.

Complete CAR data Cyt

Fig.3 Cytotoxicity assay in vitro.

CAR Construction : 2B8-CD28-CD3ζ

Fig.3 Cytotoxicity assay in vitro.

Real-time cytotoxicity assay (RTCA) showed specific killing activity of CD37-CAR-T cells against CHO-CD37 cells but not CHO cells.

CAR scFv data SPR

Fig.4 Antibody binding affinity measurements.

CAR Construction :

Fig.4 Antibody binding affinity measurements.

Surface plasmon resonance kinetic data collected on Biacore with anti-CD37 2B8 antibody bound to mouse capture chip and titration of CD37-His. KD of 1.65 nm was measured from two independent experiments.

CAR scFv data ELISA

Fig.5 2B8 specifically binds to CD37 antigen.

CAR Construction :

Fig.5 2B8 specifically binds to CD37 antigen.

ELISA shows binding of CD37 antibody 2B8D12F2D4 (2B8) clone to CD37 protein and no binding to other unrelated control proteins.

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-CD37 (2B8) h(4-1BB-CD3ζ) CAR, pCDCAR1 (CAR-0120ZP2138)

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