All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
The vector of anti-HER2 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human HER2. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-HER2 antibody linked to 4-1BB and CD3ζ signaling domains. And the vector product was designed for the treatment of Breast carcinomas.
CAR Construction : 4D5-5-41BB-CD3ζ Fig.1 Cytokine release assay. T cells were electroporated with affinity-tuned ErbB2 CAR mRNA as indicated. One day after the electroporation, the CART cells were cocultured with indicated tumor cell lines for 24 hours. B Liu, X., Jiang, S., Fang, C., Yang, S., Olalere, D., Pequignot, E. C., ... & Zhao, Y. (2015). Affinity-tuned ErbB2 or EGFR chimeric antigen receptor T cells exhibit an increased therapeutic index against tumors in mice. Cancer research, 75(17), 3596-3607. |
CAR Construction : 4D5-5-41BB-CD3ζ Fig.2 Detection of CD107a expression CD107a upregulation on CART cells stimulated by tumors. Liu, X., Jiang, S., Fang, C., Yang, S., Olalere, D., Pequignot, E. C., ... & Zhao, Y. (2015). Affinity-tuned ErbB2 or EGFR chimeric antigen receptor T cells exhibit an increased therapeutic index against tumors in mice. Cancer research, 75(17), 3596-3607. |
CAR Construction : 4D5-5-41BB-CD3ζ Fig.3 Tumor progression after the ErbB2 CART treatment. Affinity-tuned ErbB2 CART cells increase the therapeutic index and induce regression of advanced vascularized tumors in mice. Liu, X., Jiang, S., Fang, C., Yang, S., Olalere, D., Pequignot, E. C., ... & Zhao, Y. (2015). Affinity-tuned ErbB2 or EGFR chimeric antigen receptor T cells exhibit an increased therapeutic index against tumors in mice. Cancer research, 75(17), 3596-3607. |
CAR Construction : Fig.4 Comparison of measured affinities of 4D5 mutated antibodies. p185HER2ECD binding affinity of mAb 4D5 variants. Carter, P., Presta, L. E. N., Gorman, C. M., Ridgway, J. B., Henner, D., Wong, W. L., ... & Shepard, H. M. (1992). Humanization of an anti-p185HER2 antibody for human cancer therapy. Proceedings of the National Academy of Sciences, 89(10), 4285-4289. |
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