Anti-Human MUC1 (HMFG2) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF172)

Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-MUC1 (HMFG2) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human MUC1. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-MUC1 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-MUC1 (HMFG2) linked to 3nd generation CAR containing CD28, 41BB and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

Add to Cart

Description

Target
MUC1

Components
This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
1. A CAR lentiviral vector
• Anti-MUC1 (HMFG2) h(CD28-41BB-CD3ζ) CAR
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. CAR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

CAR Generation
3nd-generation CAR

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-41BB-CD3ζ

Target

Gene Name
MUC1

Full Name
mucin 1, cell surface associated

scFv
HMFG2

Host Species
Mouse

Target Species
Human

Gene ID
4582

Uniprot ID
P15941

Target Background
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Synonyms
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Related Disease
MUC1-expressing tumors

Customer Reviews and Q&As

There are currently no customer reviews or questions for Anti-Human MUC1 (HMFG2) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF172). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.