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CAR-Manu™ LDH Cytotoxicity Assay Kit (CAR-K-Z024)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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  • Size:
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  • Product Name
  • CAR-Manu™ LDH Cytotoxicity Assay Kit
  • Cat. No.
  • CAR-K-Z024
  • General Description
  • The colorimetric method was used to quantitatively determine the lactate dehydrogenase released from the damaged cells into the culture medium as a biomarker for cytotoxicity and cell lysis.
  • Product Summary
  • The LDH cytotoxicity assay kit uses an enzymatic reaction to measure extracellular LDH in the culture medium, and the red formazan produced can be measured by spectrophotometry. Lactate dehydrogenase (LDH) is a cytosolic enzyme that can indicate cytotoxicity. This assay is ideal for high-throughput screening and a safe alternative to radioactive cytotoxicity assays.

    Highlight:
    Convenient for attaching and suspending cells-add-mix-read assay format
    Colorimetry-quantitative measurement of LDH release during the formation of colored products
    Robust-use stable LDH enzyme activity as a cytotoxic marker
    Flexible-ideal for high-throughput screening
    Non-radioactive – a safe alternative to 51Cr release cytotoxicity determination
  • Components
  • Substrate mix
    Assay buffer
    10X lysis buffer
    Stop solution
    LDH positive control
  • Applications
  • Measure in vitro cytotoxicity mediated by chemicals, immune cells or siRNA or microRNA
    Detect cell lysis in bioreactors or 3D tissue engineering applications

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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