CAR-γδ T Antigen Cross-Presentation Assay Service

Antigen cross-presentation is one of the unique features of CAR-γδ T cells. As one of the top CRO companies in the field of γδ T cell therapy, Creative Biolabs provides antigen cross-presentation assays with simple and rapid methods.

Fig.1 Engineering γδ T cell with a CAR. (Rotolo, 2019)

Background

T cells of the γδ lineage are unconventional T cells and have both innate and adaptive functions and can link both responses. Similar to antigen-presenting cells (APCs) such as dendritic cells (DCs), γδ T cells show functional properties of professional antigen presentation function upon activation. Following the uptake of tumor antigen (trogocytosis, phagocytosis, or lysis), γδ T cells process and present the peptide to MHC molecule on the cell surface. Peptide-MHC complex promotes the proliferation and activation of CD4+ and CD8+ T cells. Therefore γδ T cells cross-presenting the tumor antigens at the tumor site can prolong the intra-tumoral immune response. Unlike conventional CAR-T cells, γδ T cells expressing CAR retain the ability to cross-present the tumor antigens and further improve antitumor activity.

Fig.2 Antigen presentation functions of γδ T cells. (Zou, 2017)

Our Services

• Identification of APCs markers

Activated γδ T cells possess unique and powerful antigen-presenting features, including the high expression of different APCs markers (e.g. HLA-DR, CD80, and CD86). Surface and intracellular proteins are markers that are important for identifying specific types of cells and characterizing cell populations. Traditionally, these assays are performed on flow cytometers. We can provide numerous products and services to test γδ T cells markers.

• Antigen epitopes analysis

The recognition of peptide-MHC complexes by T-cells is the cornerstone of cellular immunity. We can provide antigen epitopes analysis service. Peptides are identified by sequencing mass spectrometry.

• Antigen cross-presentation assays

Peptide-MHC complex presented by APCs promotes the proliferation and activation of T cells. γδ T-APCs are loaded with antigens coculture with responder αβ T cells and then the responses such as proliferation are analyzed to assess the function of antigen cross-presentation.

As a leader in cell therapy solutions, Creative Biolabs has extensive experience in challenging projects. If you are interested in our services, please feel free to contact us to learn more detailed information.

References

1. Rotolo, Ramona, et al. "CAR-based strategies beyond T lymphocytes: integrative opportunities for cancer adoptive immunotherapy." International Journal of Molecular Sciences 20.11 (2019): 2839. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.
2. Zou, Chang, et al. "γδ T cells in cancer immunotherapy." Oncotarget 8.5 (2016): 8900. Distributed under Open Access license CC BY 4.0, without modification.